Kumar V, Pattabiraman T N
Department of Biochemistry, S. D. M. College of Dental Sciences, 580 009 Sattur, Dharwad.
Indian J Clin Biochem. 1997 Dec;12(1):95-9. doi: 10.1007/BF02867965.
Fructose developed a pinkish orange chromogen on treatment with o-cresol: 70% sulphuric acid at 32°C for 15 minutes with a λ max of 500nm. Fructose was 185 times more chromogenic than glucose. Total carbohydrate and fructose values in protein-free filtrate of normal serum samples were in the range, 55.4-86.3 mg/dl and 1.55-3.29 mg/dl, respectively. In diabetes, the observed values were 197-354 mg/dl and 2.91-6.81 mg/dl, respectively.
果糖在用邻甲酚和70%硫酸于32℃处理15分钟后产生粉红色橙色色原，最大吸收波长为500nm。果糖的显色性比葡萄糖高185倍。正常血清样品无蛋白滤液中的总碳水化合物和果糖值分别在55.4 - 86.3mg/dl和1.55 - 3.29mg/dl范围内。在糖尿病患者中，观察到的值分别为197 - 354mg/dl和2.91 - 6.81mg/dl。
