Barichievich E M, Calza R E
Department of Animal Sciencesm Washington State University, Pullman 99164-6332.
Appl Environ Microbiol. 1990 Jan;56(1):43-8. doi: 10.1128/aem.56.1.43-48.1990.
Protein and cellulose activities were measured in culture supernatants of the anaerobic ruminal fungus Neocallimastix frontalis EB188 established in glucose medium and switched to either glucose, cellobiose, or cellulose media. Polyacrylamide gel electrophoresis was used to show differences caused by changing medium carbon source. Culture supernatants contained proteins with molecular weights ranging from greater than 116,000 to about 19,000. Low levels of cellulose activity were evident in glucose-grown cultures. Increased amounts of slowly migrating cellulase activities appeared in the supernatants of glucose-grown cultures switched to cellulose. Cellulase activities which reacted differentially during colorimetric and in situ assays were produced. Isoelectric points of cellulase activities varied from 3.7 to 8.3, and activities possessed optimal pHs of between 5.9 and 6.5.
在以葡萄糖为培养基建立并转换为葡萄糖、纤维二糖或纤维素培养基的厌氧瘤胃真菌新美鞭菌EB188的培养上清液中测定了蛋白质和纤维素活性。使用聚丙烯酰胺凝胶电泳来显示因培养基碳源变化而引起的差异。培养上清液中含有分子量范围大于116,000至约19,000的蛋白质。在葡萄糖培养的培养物中，纤维素活性水平较低。转换为纤维素的葡萄糖培养的培养物上清液中出现了数量增加的迁移缓慢的纤维素酶活性。产生了在比色法和原位测定中反应不同的纤维素酶活性。纤维素酶活性的等电点在3.7至8.3之间，活性的最佳pH值在5.9至6.5之间。
