生长在纤维素底物上的瘤胃厌氧真菌新美鞭菌的糖苷酶
Glycosidases of the rumen anaerobic fungus Neocallimastix frontalis grown on cellulosic substrates.
作者信息
Pearce P D, Bauchop T
出版信息
Appl Environ Microbiol. 1985 May;49(5):1265-9. doi: 10.1128/aem.49.5.1265-1269.1985.
Abstract
The rumen anaerobic fungus Neocallimastix frontalis was grown on cellulosic substrates, and the cellular distribution and types of glycosidases produced by the organism were studied. Fungal cultures were fractionated into extracellular, insoluble (membrane), and intracellular fractions and assayed for glycosidase activity by using Avicel, carboxymethylcellulose, xylan, starch, polygalacturonic acid, and the p-nitrophenyl derivatives of galactose, glucose, and xylose as substrates. Enzymic activity was highest in the extracellular fraction; however, the membrane fraction also displayed appreciable activity. The intracellular fraction was inactive towards all substrates. Polygalacturonic acid was the only substrate not hydrolyzed by the active fractions, indicating that pectinase was absent. The results show that N. frontalis, a common rumen anaerobic fungus, produces enzymes for degrading cellulose and hemicellulose, key components of plant fiber.
摘要
瘤胃厌氧真菌新美鞭菌（Neocallimastix frontalis）在纤维素底物上生长，并对该生物体产生的糖苷酶的细胞分布和类型进行了研究。将真菌培养物分为细胞外、不溶性（膜）和细胞内部分，以微晶纤维素、羧甲基纤维素、木聚糖、淀粉、聚半乳糖醛酸以及半乳糖、葡萄糖和木糖的对硝基苯基衍生物作为底物，测定糖苷酶活性。酶活性在细胞外部分最高；然而，膜部分也表现出可观的活性。细胞内部分对所有底物均无活性。聚半乳糖醛酸是唯一不被活性部分水解的底物，表明不存在果胶酶。结果表明，常见的瘤胃厌氧真菌新美鞭菌产生用于降解植物纤维关键成分纤维素和半纤维素的酶。
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