酶联蛋白A：一种用于检测人免疫球蛋白G和病毒特异性抗体的酶联免疫吸附测定试剂。
Enzyme-linked protein A: an enzyme-linked immunosorbent assay reagent for detection of human immunoglobulin G and virus-specific antibody.
作者信息
Madore H P, Baumgarten A
出版信息
J Clin Microbiol. 1979 Oct;10(4):529-32. doi: 10.1128/jcm.10.4.529-532.1979.
Abstract
A general-purpose reagent capable of reacting with immunoglobulin G in a modified enzyme-linked immunosorbent assay technique was prepared by using protein A coupled with horseradish peroxidase. The reagent detected low levels (0.003 to 1.0 microgram/ml) of human immunoglobulin G and was also applied in an enzyme-linked immunosorbent assay for titration of antibody to human cytomegalovirus. The antibody titers to human cytomegalovirus determined by enzyme-linked immunosorbent assay and by complement fixation were compared. The correlation coefficient between the two techniques was 0.85, but the enzyme-linked immunosorbent assay was 10 times more sensitive than complement fixation in terms of antibody titers detected.
摘要
通过将蛋白A与辣根过氧化物酶偶联，制备了一种在改良酶联免疫吸附测定技术中能够与免疫球蛋白G发生反应的通用试剂。该试剂可检测低水平（0.003至1.0微克/毫升）的人免疫球蛋白G，并且还应用于酶联免疫吸附测定中以滴定抗人巨细胞病毒抗体。比较了通过酶联免疫吸附测定和补体结合法测定的抗人巨细胞病毒抗体滴度。两种技术之间的相关系数为0.85，但就检测到的抗体滴度而言，酶联免疫吸附测定比补体结合法灵敏10倍。
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本文引用的文献
1
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Infect Immun. 1970 Nov;2(5):672-3. doi: 10.1128/iai.2.5.672-673.1970.
2
Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes.酶联免疫吸附测定。二、借助酶标记抗原和抗体包被管对蛋白质抗原、免疫球蛋白G进行定量测定。
Biochim Biophys Acta. 1971 Dec 28;251(3):427-34. doi: 10.1016/0005-2795(71)90132-2.
3
Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration.过氧化物酶标记抗体及具有增强细胞内穿透能力的Fab缀合物。
Immunochemistry. 1971 Dec;8(12):1175-9. doi: 10.1016/0019-2791(71)90395-8.
4
Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.酶联免疫吸附测定（ELISA）。免疫球蛋白G的定量测定。
Immunochemistry. 1971 Sep;8(9):871-4. doi: 10.1016/0019-2791(71)90454-x.
5
Differences in anti-protein A activity among IgG subgroups.IgG亚组间抗蛋白A活性的差异。
J Immunol. 1969 Oct;103(4):828-33.
6
7
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Eur J Biochem. 1972 Sep 25;29(3):572-8. doi: 10.1111/j.1432-1033.1972.tb02023.x.
8
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J Histochem Cytochem. 1974 Dec;22(12):1084-91. doi: 10.1177/22.12.1084.
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