Madore H P, Baumgarten A
J Clin Microbiol. 1979 Oct;10(4):529-32. doi: 10.1128/jcm.10.4.529-532.1979.
A general-purpose reagent capable of reacting with immunoglobulin G in a modified enzyme-linked immunosorbent assay technique was prepared by using protein A coupled with horseradish peroxidase. The reagent detected low levels (0.003 to 1.0 microgram/ml) of human immunoglobulin G and was also applied in an enzyme-linked immunosorbent assay for titration of antibody to human cytomegalovirus. The antibody titers to human cytomegalovirus determined by enzyme-linked immunosorbent assay and by complement fixation were compared. The correlation coefficient between the two techniques was 0.85, but the enzyme-linked immunosorbent assay was 10 times more sensitive than complement fixation in terms of antibody titers detected.
通过将蛋白A与辣根过氧化物酶偶联，制备了一种在改良酶联免疫吸附测定技术中能够与免疫球蛋白G发生反应的通用试剂。该试剂可检测低水平（0.003至1.0微克/毫升）的人免疫球蛋白G，并且还应用于酶联免疫吸附测定中以滴定抗人巨细胞病毒抗体。比较了通过酶联免疫吸附测定和补体结合法测定的抗人巨细胞病毒抗体滴度。两种技术之间的相关系数为0.85，但就检测到的抗体滴度而言，酶联免疫吸附测定比补体结合法灵敏10倍。
