Reitano M, Pisano M A, Eriquez L A, D'Amato R F
J Clin Microbiol. 1986 Jan;23(1):62-5. doi: 10.1128/jcm.23.1.62-65.1986.
An enzyme-linked immumosorbent assay (ELISA), based upon the detection of streptolysin O antibodies in human sera, was developed. Disposable polystyrene tubes, sensitized with streptolysin O antigen, were used as the test vehicles. Corresponding antibodies, present in test sera, were detected by binding of the antibodies to goat anti-human immunoglobulin G conjugated to horseradish peroxidase. Demonstration of bound conjugate was accomplished by monitoring peroxidase activity spectrophotometrically at 450 nm, using 5-aminosalicylic acid as the indicator. A total of 97 human sera, previously analyzed by means of the anti-streptolysin O titration technique, were evaluated with the ELISA procedure. A direct quantitative relationship, found to be statistically significant, was demonstrated between Todd units and absorbance values obtained with ELISA.
开发了一种基于检测人血清中抗链球菌溶血素O抗体的酶联免疫吸附测定（ELISA）。用链球菌溶血素O抗原致敏的一次性聚苯乙烯管用作检测载体。通过将抗体与与辣根过氧化物酶偶联的山羊抗人免疫球蛋白G结合来检测测试血清中存在的相应抗体。通过使用5-氨基水杨酸作为指示剂在450nm处分光光度法监测过氧化物酶活性来完成结合的缀合物的检测。用ELISA程序评估了总共97份先前通过抗链球菌溶血素O滴定技术分析的人血清。发现Todd单位与ELISA获得的吸光度值之间存在统计学上显著的直接定量关系。
