State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, China.
Molecules. 2012 Oct 29;17(11):12746-57. doi: 10.3390/molecules171112746.
To elucidate the underlying mechanism of cardio-protective activity of the total ginsenosides (TGS) of Radix Ginseng, proteomic analysis using two-dimensional gel electrophoresis (2-DE) and MALDI-TOF-TOF-MS techniques was employed for identifying the underlying targets of TGS on improvement of the energy metabolism of isolated rat heart tissues perfused in Langendorff system under ischemia-reperfusion injury conditions. The image analysis results revealed 11 differentially expressed proteins in the TGS-treated heart tissues; these proteins, including LDHB and ODP-2, were found to be closely related to the function of tricarboxylic acid (TCA) cycle that plays pivotal roles in cardiac energy metabolism. It is thus concluded that improvement of cardiac energy metabolism via activating proteins in TCA cycle could be the major action pathway and targets of TGS activity against rat heart tissue injury.
为了阐明人参总皂苷（TGS）的心脏保护作用的潜在机制，采用二维凝胶电泳（2-DE）和 MALDI-TOF-TOF-MS 技术的蛋白质组学分析方法，鉴定 TGS 在改善缺血再灌注损伤条件下 Langendorff 系统灌流分离大鼠心脏组织能量代谢方面的潜在作用靶点。图像分析结果显示，TGS 处理的心脏组织中有 11 种差异表达的蛋白质；这些蛋白质，包括 LDHB 和 ODP-2，与三羧酸（TCA）循环的功能密切相关，TCA 循环在心脏能量代谢中起着关键作用。因此，可以得出结论，通过激活 TCA 循环中的蛋白质来改善心脏能量代谢可能是 TGS 活性对抗大鼠心脏组织损伤的主要作用途径和靶点。
