European School of Molecular Medicine (SEMM), IFOM-IEO, Milano, Italy.
Nanotechnology. 2012 Nov 30;23(47):475101. doi: 10.1088/0957-4484/23/47/475101. Epub 2012 Oct 30.
Nanostructured cubic zirconia is a strategic material for biomedical applications since it combines superior structural and optical properties with a nanoscale morphology able to control cell adhesion and proliferation. We produced nanostructured cubic zirconia thin films at room temperature by supersonic cluster beam deposition of nanoparticles produced in the gas phase. Precise control of film roughness at the nanoscale is obtained by operating in a ballistic deposition regime. This allows one to study the influence of nanoroughness on cell adhesion, while keeping the surface chemistry constant. We evaluated cell adhesion on nanostructured zirconia with an osteoblast-like cell line using confocal laser scanning microscopy for detailed morphological and cytoskeleton studies. We demonstrated that the organization of cytoskeleton and focal adhesion formation can be controlled by varying the evolution of surface nanoroughness.
纳米结构立方氧化锆是一种用于生物医学应用的战略性材料，因为它结合了卓越的结构和光学性能，以及纳米级形态，能够控制细胞黏附和增殖。我们通过超音速团束沉积气相中产生的纳米颗粒，在室温下制备了纳米结构立方氧化锆薄膜。通过在弹道沉积模式下工作，可以精确控制薄膜的纳米级粗糙度。这使得人们可以在保持表面化学性质不变的情况下，研究纳米粗糙度对细胞黏附的影响。我们使用共聚焦激光扫描显微镜，通过成骨样细胞系评估了纳米结构氧化锆上的细胞黏附情况，进行了详细的形态学和细胞骨架研究。我们证明，通过改变表面纳米粗糙度的演变，可以控制细胞骨架的组织和焦点黏附的形成。
