Distribution of Elements and Presence of Panton-Valentine Leukocidin in Methicillin-Resistant Isolated from Clinical Samples in a University Hospital of Isfahan City, Iran.

INTRODUCTION

Coagulase Negative (CoNS) is considered as a major pathogen of nosocomial infections among immunosuppressed patients.

AIM

The aim of this study was to identify the types of () and Panton-Valentine Leukocidin () gene among clinical Methicillin-Resistant isolates collected from Isfahan.

MATERIALS AND METHODS

This cross-sectional study was performed from March 2014 to January 2015 at a tertiary care hospital of Isfahan, Iran. Antimicrobial susceptibility tests of isolates were performed by the disc diffusion method. All the strains were screened for methicillin resistance based on resistance to cefoxitin (30 μg) disc and presence of gene. Determination of typing and toxin gene were performed by PCR method. For categorical variables different groups were compared using the Chi-square test or Fisher exact test. A p-value of <0.05 was considered significant for all statistical tests.

RESULTS

The frequency of MRSE was 53.8% according to the presence of gene. The overall resistance rate was high with ciprofloxacin (81.4%). PCR analysis showed that 17% (12/70) of MRSE isolate carried the PVL gene and 43% (30/70) were type I; 11.4% (8/70) were type II; and 34.2% (24/70) were type IV, whereas, 11.4% (8/70) of the MRSE isolates could not be typed.

CONCLUSION

type I was the major type of , which indicates an emergence of this type in the studied medical centers. Increased prevalence of types in community is cause of an increase in antibiotic resistance among microorganisms.