Al-Mariri Ayman, Saour George, Hamou Razan
Department of Molecular Biology and Biotechnology, Atomic Energy Commission, Damascus, Syria.
Iran J Med Sci. 2012 Jun;37(2):119-25.
Brucellaabortus is a gram-negative facultative intracellular bacterium that can cause a highly contagious disease in sheep, goats, cattle and one-humped camels. It is responsible for one of the most important zoonosis in human. The aim of this study was to evaluate the role of Mentha piperita, Origanum majorana, Citrus lemon, Cinnamomum verum and Myristica fragrans essential volatile oil extracts on human macrophages infected by B. abortus 544.
Essential volatile oil extracts from M. piperita, O. majorana, C. lemon, C. verum and M. fragrans were extracted. Human macrophages were cultured at a density of 2×10(5) cells per well in sterile 96-well microtiter plates, and infected with B. abortus 544 at a ratio of 1:100 bacteria/cell. Then essential volatile oil extracts were added at a concentration of 1%. At specified times; cells were washed, lysed with 0.1% Triton, and plated on 2YT agar to determine the number of intracellular bacteria.
Cinnamomum verum volatile oil at a concentration of 1% had the highest antibacterial activity against B. abortus 544 inside human macrophages. Its inhibitory effect observed from 24 h and continued till 144 h after the infection. Moreover, C. verum (0.1%) in combination with 1% concentration of M. piperita, O. majorana, C. lemon or M. fragrans volatile oil extracts produced a synergistic inhibitory effect against B. abortus 544.
The results indicate that, among the five selected oil extracts, C. verum volatile oil applied either separately or in combination with other oil extracts had the most effective antimicrobial activity against Brucella.
流产布鲁氏菌是一种革兰氏阴性兼性细胞内细菌，可在绵羊、山羊、牛和单峰骆驼中引发高度传染性疾病。它是人类最重要的人畜共患病之一的病原体。本研究的目的是评估薄荷、马郁兰、柠檬、肉桂和肉豆蔻的挥发油提取物对感染流产布鲁氏菌544的人类巨噬细胞的作用。
提取薄荷、马郁兰、柠檬、肉桂和肉豆蔻的挥发油提取物。将人类巨噬细胞以每孔2×10⁵个细胞的密度接种于无菌96孔微量滴定板中，并以1:100的细菌/细胞比例感染流产布鲁氏菌544。然后以1%的浓度添加挥发油提取物。在特定时间；洗涤细胞，用0.1% Triton裂解，并接种于2YT琼脂上以确定细胞内细菌的数量。
浓度为1%的肉桂挥发油对人类巨噬细胞内的流产布鲁氏菌544具有最高的抗菌活性。在感染后24小时观察到其抑制作用，并持续至144小时。此外，0.1%的肉桂与1%浓度的薄荷、马郁兰、柠檬或肉豆蔻挥发油提取物联合使用对流产布鲁氏菌544产生协同抑制作用。
结果表明，在所选的五种油提取物中，单独使用或与其他油提取物联合使用的肉桂挥发油对布鲁氏菌具有最有效的抗菌活性。
