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银纳米颗粒对巨噬细胞内流产布鲁氏菌544的杀菌作用

Bactericidal Effect of Silver Nanoparticles on Intramacrophage Brucella abortus 544.

作者信息

Alizadeh Hamed, Salouti Mojtaba, Shapouri Reza

机构信息

Young Researchers and Elite Club, Zanjan Branch, Islamic Azad University, Zanjan, IR Iran.

Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, IR Iran.

出版信息

Jundishapur J Microbiol. 2014 Mar;7(3):e9039. doi: 10.5812/jjm.9039. Epub 2014 Mar 1.

DOI:10.5812/jjm.9039
PMID:25147682
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4138654/
Abstract

BACKGROUND

Brucellosis is an infectious disease that is caused by Brucella spp. As Brucella spp. are intramacrophage pathogens, the treatment of this infection is very difficult. On the other hand, due to the side effects of the brucellosis treatment regime, it is necessary to find new antimicrobial agents against it.

OBJECTIVES

The aim of this study was to investigate the antimicrobial effect of silver nanoparticles against Brucella abortus 544 in the intramacrophage condition.

MATERIALS AND METHODS

The antimicrobial effect of silver nanoparticles was determined by an agar well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against B. abortus 544 were determined by a broth macrodilution method. The effect of time on the antimicrobial activity of silver nanoparticles was analyzed. The effect of silver nanoparticles on the intramacrophage survival of B. abortus 544 was studied on mice peritoneal macrophages.

RESULTS

The well diffusion agar study showed that silver nanoparticles have an antimicrobial effect on B. abortus 544. The MIC and MBC of silver nanoparticles against B. abortus 544 were; 6 ppm and 8 ppm, respectively. The silver nanoparticles showed antibacterial effects within 40 minutes. The results of the macrophage culture indicated that silver nanoparticles have antibacterial activity against intramacrophage B. abortus 544, and the highest efficiency was observed at a concentration of 8-10 ppm of silver nanoparticles.

CONCLUSIONS

The results showed that silver nanoparticles have an antimicrobial effect against intramacrophage B. abortus 544.

摘要

背景

布鲁氏菌病是由布鲁氏菌属引起的一种传染病。由于布鲁氏菌属是巨噬细胞内病原体，这种感染的治疗非常困难。另一方面，由于布鲁氏菌病治疗方案的副作用，有必要寻找针对它的新型抗菌剂。

目的

本研究旨在调查银纳米颗粒在巨噬细胞内条件下对流产布鲁氏菌544的抗菌作用。

材料与方法

采用琼脂孔扩散法测定银纳米颗粒的抗菌作用。采用肉汤稀释法测定银纳米颗粒对流产布鲁氏菌544的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。分析时间对银纳米颗粒抗菌活性的影响。在小鼠腹腔巨噬细胞上研究银纳米颗粒对流产布鲁氏菌544在巨噬细胞内存活的影响。

结果

琼脂孔扩散研究表明，银纳米颗粒对流产布鲁氏菌544有抗菌作用。银纳米颗粒对流产布鲁氏菌544的MIC和MBC分别为6 ppm和8 ppm。银纳米颗粒在40分钟内显示出抗菌效果。巨噬细胞培养结果表明，银纳米颗粒对巨噬细胞内的流产布鲁氏菌544有抗菌活性，在银纳米颗粒浓度为8 - 10 ppm时观察到最高效率。

结论

结果表明，银纳米颗粒对巨噬细胞内的流产布鲁氏菌544有抗菌作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7eb9/4138654/b12df280b9d3/jjm-07-9039-i001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7eb9/4138654/b12df280b9d3/jjm-07-9039-i001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7eb9/4138654/b12df280b9d3/jjm-07-9039-i001.jpg

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