The University of Reading, Food and Nutritional Sciences, Whiteknights, PO Box 226, Reading RG6 6AP, United Kingdom.
J Proteome Res. 2012 Dec 7;11(12):5573-85. doi: 10.1021/pr300637d. Epub 2012 Nov 1.
Microbial metabolism of proteins and amino acids by human gut bacteria generates a variety of compounds including phenol, indole, and sulfur compounds and branched chain fatty acids, many of which have been shown to elicit a toxic effect on the lumen. Bacterial fermentation of amino acids and proteins occurs mainly in the distal colon, a site that is often fraught with symptoms from disorders including ulcerative colitis (UC) and colorectal cancer (CRC). In contrast to carbohydrate metabolism by the gut microbiota, proteolysis is less extensively researched. Many metabolites are low molecular weight, volatile compounds. This review will summarize the use of analytical methods to detect and identify compounds in order to elucidate the relationship between specific dietary proteinaceous substrates, their corresponding metabolites, and implications for gastrointestinal health.
人肠道细菌对蛋白质和氨基酸的微生物代谢会产生多种化合物，包括酚、吲哚和硫化合物以及支链脂肪酸，其中许多已被证明对腔有毒性作用。细菌对氨基酸和蛋白质的发酵主要发生在远端结肠，这个部位常常受到溃疡性结肠炎（UC）和结直肠癌（CRC）等疾病的困扰。与肠道微生物群的碳水化合物代谢相比，蛋白质水解的研究还不够广泛。许多代谢物是低分子量、挥发性化合物。这篇综述将总结分析方法的使用，以检测和鉴定化合物，以阐明特定膳食蛋白质底物、其相应代谢物与胃肠道健康之间的关系。
