嗜肺军团菌巴里2/158株细胞脂肪酸组成的重新评估
Reevaluation of the cellular fatty acid composition of Legionella micdadei Bari 2/158.
作者信息
Moss C W, Lambert-Fair M A
机构信息
Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
出版信息
J Clin Microbiol. 1990 Feb;28(2):389-90. doi: 10.1128/jcm.28.2.389-390.1990.
The cellular fatty acid composition of Legionella micdadei Bari 2/158 was reevaluated because of its purported differences from other L. micdadei strains and its similarity to L. bozemanii. We found the fatty acid content of this strain to be consistent with that of 11 other strains of L. micdadei, including the presence of an anteiso branched-chain, monounsaturated, 17-carbon acid (Ca17:1) which is characteristic of this species. The double-bond position of Ca17:1 was established at the omega 7 (or delta 9) position by combined gas chromatographic-mass spectrometric analysis of dimethyl disulfide derivatives. The Ca17:1 omega 7 acid was absent in each of 14 strains of L. bozemanii.
由于据称米克戴德军团菌巴里2/158株的细胞脂肪酸组成与其他米克戴德军团菌菌株存在差异，且与博兹曼军团菌相似，因此对其进行了重新评估。我们发现该菌株的脂肪酸含量与其他11株米克戴德军团菌一致，包括存在该物种特有的anteiso支链单不饱和17碳脂肪酸（Ca17:1）。通过对二甲基二硫衍生物的气相色谱 - 质谱联用分析，确定Ca17:1的双键位置在ω7（或δ9）位。在14株博兹曼军团菌的每一株中均未检测到Ca17:1 ω7酸。
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