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在黑叉尾斗鱼和其他虾虎鱼物种(鲈形目,虾虎鱼科)中分离新型微卫星位点。

Isolation of novel microsatellite loci in the black goby Gobius niger and cross-amplification in other gobiid species (Perciformes, Gobiidae).

机构信息

Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Ancona 60131, Italy.

出版信息

J Fish Biol. 2012 Nov;81(6):2044-52. doi: 10.1111/j.1095-8649.2012.03444.x. Epub 2012 Oct 10.

DOI:10.1111/j.1095-8649.2012.03444.x
PMID:23130698
Abstract

Twelve microsatellite loci were isolated from and characterized for the black goby Gobius niger. These loci were tested on a total of 48 individuals from two geographically distant locations (Orbetello and Cattolica) and the number of alleles ranged from two to 18, with expected (H(e)) and observed (H(o)) heterozygosities ranging from 0.042 to 0.941 and from 0.042 to 0.917, respectively. The loci described were used to cross-amplify other gobiid species belonging to Gobius, Zosterisessor, Lesueurigobius and Aphia.

摘要

从黑鲷鱼(Gobius niger)中分离并鉴定了 12 个微卫星基因座。这些基因座在两个地理位置相距较远的地点(奥尔贝泰洛和卡托利卡)的 48 个个体上进行了测试,等位基因数从 2 到 18 不等,预期杂合度(H(e))和观察杂合度(H(o))分别为 0.042 至 0.941 和 0.042 至 0.917。所描述的基因座用于扩增属于 Gobius、Zosterisessor、Lesueurigobius 和 Aphia 的其他虾虎鱼物种。

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