采用乳胶凝集法检测粪便样本中的41型腺病毒。

Detection of adenovirus type41 in stool samples by a latex agglutination method.

作者信息

Sanekata T, Taniguchi K, Demura M, Fujinaga K

机构信息

Department of Veterinary Microbiology, Faculty of Agriculture, Tottori University, Japan.

出版信息

J Immunol Methods. 1990 Mar 9;127(2):235-9. doi: 10.1016/0022-1759(90)90073-5.

DOI:10.1016/0022-1759(90)90073-5

PMID:2313101

Abstract

We have developed a simple agglutination (LA) method for the detection of enteric adenovirus (EAd) in stool samples from infants with acute gastroenteritis. Ad type 41 (Ad41) was detected with high sensitivity and specificity by a slide agglutination test using latex particles coated with antiAd41 antibody (LA-antiAd41). The agglutination of LA-antiAd41 with Ad41 on a glass slide was evident macroscopically within 2 min. The sensitivity of the LA method was four times higher than that of the EM method.

摘要

我们开发了一种简单的凝集试验（LA），用于检测急性胃肠炎婴儿粪便样本中的肠道腺病毒（EAd）。通过使用包被有抗Ad41抗体的乳胶颗粒的玻片凝集试验（LA-antiAd41），以高灵敏度和特异性检测到了41型腺病毒（Ad41）。在玻片上，LA-antiAd41与Ad41的凝集在2分钟内肉眼可见。LA方法的灵敏度比电子显微镜（EM）方法高四倍。