Grandien M, Pettersson C A, Svensson L, Uhnoo I
Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
J Med Virol. 1987 Dec;23(4):311-6. doi: 10.1002/jmv.1890230402.
A commercial latex agglutination test for diagnosis of adenovirus in diarrheal disease (Adenolex, Orion Diagnostica, Finland) was evaluated by comparison with the results obtained by ELISA, electron microscopy (EM), and virus isolation. Fifty specimens originated from the diagnostic routine, and 50 were selected from a previous epidemiological study on the etiology of diarrheal disease in children. Thirteen of the 100 specimens reacted with the latex control, impairing interpretation of the results. Although the ELISA detected adenovirus antigen in 10(2) higher dilutions than the latex agglutination test, a total agreement was obtained between results by the two tests for 87 specimens including 42 positives. The two additional positives found by EM and virus isolation could not be diagnosed by the latex agglutination test. Of 37 specimens containing enteric adenoviruses (types 40 and 41), the agglutination test diagnosed all but 4 specimens containing type 41 virus. These four specimens were negative also by ELISA and adenovirus had been detected by virus isolation on the 293 cell line. The latex agglutination test gave positive results with nine specimens containing adenovirus types other than the enteric types 40 and 41. The latex agglutination test was found to be a rapid and simple method for the detection of adenovirus in diarrheal disease. Compared to ELISA and EM, the sensitivity was 100% and 95% respectively, and the specificity 100%.
通过与酶联免疫吸附测定（ELISA）、电子显微镜检查（EM）和病毒分离所获得的结果进行比较，对一种用于诊断腹泻病中腺病毒的商业乳胶凝集试验（Adenolex，芬兰奥立安诊断公司）进行了评估。50份标本来自诊断常规流程，另外50份则是从先前一项关于儿童腹泻病病因的流行病学研究中选取的。100份标本中有13份与乳胶对照发生反应，影响了结果的判读。尽管ELISA检测腺病毒抗原的稀释度比乳胶凝集试验高10²倍，但在包括42份阳性标本在内的87份标本中，两种检测方法的结果完全一致。EM和病毒分离检测出的另外两份阳性标本，乳胶凝集试验无法诊断。在37份含有肠道腺病毒（40型和41型）的标本中，凝集试验除了4份含有41型病毒的标本外，诊断出了所有其他标本。这4份标本ELISA检测也呈阴性，且在293细胞系上通过病毒分离检测到了腺病毒。乳胶凝集试验对9份含有除肠道40型和41型以外腺病毒类型的标本给出了阳性结果。结果发现，乳胶凝集试验是一种检测腹泻病中腺病毒的快速简便方法。与ELISA和EM相比，其灵敏度分别为100%和95%，特异性为100%。
