Laboratoire de Biotechnologies Végétales Appliquées aux Plantes Aromatiques et Médicinales, Université Jean Monnet, Université de Lyon, Saint-Etienne, France.
PLoS One. 2012;7(10):e48253. doi: 10.1371/journal.pone.0048253. Epub 2012 Oct 25.
Sclareol is a high-value natural product obtained by solid/liquid extraction of clary sage (Salvia sclarea L.) inflorescences. Because processes of excretion and accumulation of this labdane diterpene are unknown, the aim of this work was to gain knowledge on its sites of accumulation in planta. Samples were collected in natura or during different steps of the industrial process of extraction (steam distillation and solid/liquid extraction). Samples were then analysed with a combination of complementary analytical techniques (gas chromatography coupled to a mass spectrometer, polarized light microscopy, environmental scanning electron microscopy, two-photon fluorescence microscopy, second harmonic generation microscopy). According to the literature, it is hypothesized that sclareol is localized in oil pockets of secretory trichomes. This study demonstrates that this is not the case and that sclareol accumulates in a crystalline epicuticular form, mostly on calyces.
薰衣草醇是一种高附加值的天然产物，通过对快乐鼠尾草（Salvia sclarea L.）花序进行固/液萃取获得。由于该类贝壳杉烷二萜的排泄和积累过程尚不清楚，因此本工作旨在了解其在植物体内的积累部位。采集了天然样品或在提取（蒸汽蒸馏和固/液萃取）的不同工业步骤过程中的样品。然后使用多种互补分析技术（气相色谱-质谱联用、偏光显微镜、环境扫描电子显微镜、双光子荧光显微镜、二次谐波产生显微镜）对样品进行分析。根据文献，推测薰衣草醇存在于分泌性毛状体的油囊中。本研究表明，事实并非如此，薰衣草醇以结晶的外角质层形式积累，主要在花萼上。
