高分辨率分子成像技术通过活体显微镜实现：体内血管生物学的照亮。
High-resolution molecular imaging via intravital microscopy: illuminating vascular biology in vivo.
机构信息
Cardiovascular Division, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02114, USA.
出版信息
Integr Biol (Camb). 2013 Feb;5(2):278-90. doi: 10.1039/c2ib20194a.
Abstract
Complications of atherosclerosis and thrombosis are leading causes of death worldwide. While experimental investigations have yielded valuable insights into key molecular and cellular phenomena in these diseases of medium- and large-sized vessels, direct visualization of relevant in vivo biological processes has been limited. However, recent developments in molecular imaging technology, specifically fluorescence imaging agents coupled with high-resolution, high-speed intravital microscopy (IVM), are now enabling dynamic and longitudinal investigations into the mechanisms and progression of many vascular diseases. Here we review recent advances in IVM that have provided new in vivo biological insights into atherosclerosis and thrombosis.
摘要
动脉粥样硬化和血栓形成的并发症是全球范围内主要的死亡原因。虽然实验研究为中大型血管疾病中的关键分子和细胞现象提供了有价值的见解，但对相关体内生物过程的直接观察一直受到限制。然而，分子成像技术的最新发展，特别是与高分辨率、高速活体显微镜（IVM）结合的荧光成像剂，现在使我们能够对许多血管疾病的机制和进展进行动态和纵向研究。在这里，我们回顾了 IVM 的最新进展，这些进展为动脉粥样硬化和血栓形成提供了新的体内生物学见解。
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