Department of Research & Technology Development, JGreen Inc., Jinju 660-841, Republic of Korea.
Division of Applied Life Science (BK21), Plant Molecular Biology & Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Jun;63(Pt 6):2277-2281. doi: 10.1099/ijs.0.045484-0. Epub 2012 Nov 16.
A Gram-negative, aerobic, short rod-shaped bacterium, designated strain YC6898(T), was isolated from the surface-sterilized root of a halophyte (Suaeda maritima) inhabiting tidal flat of Namhae Island, Korea. Strain YC6898(T) grew optimally at 30-37 °C and pH 6.5-7.5. The strain inhibited mycelial growth of Pythium ultimum and Phytophthora capsici. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YC6898(T) belongs to the genus Hoeflea in the family Phyllobacteriaceae. Its closest relatives were Hoeflea alexandrii AM1V30(T) (96.7% 16S rRNA gene sequence similarity), Hoeflea anabaenae WH2K(T) (95.7%), Hoeflea phototrophica DFL-43(T) (95.5%) and Hoeflea marina LMG 128(T) (94.8%). Strain YC6898(T) contained Q-10 as the major ubiquinone. The major fatty acids of strain YC6898(T) were C18:1ω7c (61.1%), C16:0 (11.9%), 11-methyl C18:1ω7c (9.6%) and C19:0 cyclo ω8c (8.0%). The polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, unknown lipids and an unknown glycolipid. The total genomic DNA G+C content was 53.7 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain YC6898(T) represents a novel species of the genus Hoeflea, for which the name Hoeflea suaedae sp. nov. is proposed. The type strain is YC6898(T) (=KACC 14911(T)=NBRC 107700(T)).
一株革兰氏阴性、需氧、短杆状细菌，被命名为YC6898(T) 菌株，从韩国南海岛潮汐滩生耐盐植物（盐地碱蓬）的表面灭菌根部分离得到。YC6898(T) 菌株在 30-37°C 和 pH6.5-7.5 条件下生长最佳。该菌株能抑制腐霉菌和辣椒疫霉的菌丝生长。基于 16S rRNA 基因序列的系统发育分析表明，YC6898(T) 菌株属于黄杆菌科 Hoeflea 属。其最接近的亲缘种为亚历山大 Hoeflea alexandrii AM1V30(T)（96.7% 16S rRNA 基因序列相似性）、鱼腥藻 Hoeflea anabaenae WH2K(T)（95.7%）、光养型 Hoeflea phototrophica DFL-43(T)（95.5%）和海生 Hoeflea marina LMG 128(T)（94.8%）。YC6898(T) 菌株含有 Q-10 作为主要的泛醌。YC6898(T) 菌株的主要脂肪酸为 C18:1ω7c（61.1%）、C16:0（11.9%）、11-甲基 C18:1ω7c（9.6%）和 C19:0 环 ω8c（8.0%）。极性脂为磷脂酰胆碱、磷脂酰乙醇胺、磷脂单甲基乙醇胺、磷脂酰甘油、未知脂类和未知糖脂。总基因组 DNA G+C 含量为 53.7 mol%。基于表型、化学分类和系统发育分析，YC6898(T) 菌株代表了黄杆菌属的一个新种，命名为 Hoeflea suaedae sp. nov.。模式菌株为 YC6898(T)（=KACC 14911(T)=NBRC 107700(T)）。
