Department of Microbiology, Chungbuk National University, Cheongju, 361-763, Republic of Korea.
Antonie Van Leeuwenhoek. 2013 May;103(5):971-8. doi: 10.1007/s10482-013-9876-6. Epub 2013 Jan 12.
A Gram-negative, aerobic, motile, straight or curved rod-shaped marine bacterium was isolated from marine sediment of the East Sea, Korea. The isolated strain, JG120-1(T), grows with 0-5 % (w/v) NaCl and at 15-30 °C and pH 6-9. α-galactosidase activity test was positive. Comparative 16S rRNA gene sequence studies showed that this strain belonged to the Alphaproteobacteria and was the most closely related to Hoeflea alexandrii AM1 V30(T), Hoeflea phototrophica DFL-43(T) and Hoeflea marina LMG 128(T) (98.9, 97.9 and 97.0 % 16S rRNA gene sequence similarities, respectively). Strain JG120-1(T) was found to possess summed feature 8 (C18:1ω7c/C18:1ω6c, 71.11 %) as the major cellular fatty acid. The major ubiquinone was determined to be Q-10. Polar lipids include phosphatidylglycerol, phosphatidylethanolamine, sulfoquinovosyl diacylglycerol, phosphatidylcholine and phosphatidylmonomethylethanolamine. The G+C content of the genomic DNA of strain JG120-1(T) was determined to be 57.8 mol %. DNA-DNA relatedness data indicated that strain JG120-1(T) represents a distinct species that is separate from H. phototrophica DFL-43(T), H. marina LMG128(T) and H. alexandrii AM1 V30(T). On the basis of polyphasic evidences, it is proposed that strain JG120-1(T) (= KCTC 23107(T) = JCM 16715(T)) represents the type strain of a novel species, Hoeflea halophila sp. nov.
一株革兰氏阴性、需氧、运动、直或弯的杆状海洋细菌，从韩国东海海洋沉积物中分离得到。分离株 JG120-1(T) 在 0-5%（w/v）NaCl 浓度、15-30°C 和 pH6-9 的条件下生长。α-半乳糖苷酶活性试验阳性。比较 16S rRNA 基因序列研究表明，该菌株属于α-变形菌门，与 Hoeflea alexandrii AM1 V30(T)、Hoeflea phototrophica DFL-43(T) 和 Hoeflea marina LMG 128(T)最为密切相关（16S rRNA 基因序列相似度分别为 98.9%、97.9%和 97.0%）。发现菌株 JG120-1(T) 主要细胞脂肪酸为 summed feature 8（C18:1ω7c/C18:1ω6c，71.11%）。主要醌类为 Q-10。极性脂包括磷脂酰甘油、磷脂酰乙醇胺、磺基海藻糖二酰甘油、磷脂酰胆碱和磷脂单甲醚乙醇胺。菌株 JG120-1(T) 的基因组 DNA G+C 含量为 57.8mol%。DNA-DNA 同源性数据表明，菌株 JG120-1(T) 代表一种与 H. phototrophica DFL-43(T)、H. marina LMG128(T) 和 H. alexandrii AM1 V30(T) 不同的独特物种。基于多相证据，建议菌株 JG120-1(T)（=KCTC 23107(T)=JCM 16715(T)）代表一个新种的模式株，Hoeflea halophila sp. nov.。
