Department of Molecular and Human Genetics, College of Medicine, Florida International University, Miami, FL 33199, USA.
Electrophoresis. 2012 Dec;33(23):3555-63. doi: 10.1002/elps.201200361.
In the present study, 17 Y-chromosomal STR (Y-STR) loci were typed in 218 unrelated males from Sousse, Central-East Tunisia, to evaluate forensic and population genetic applications of the data. A total of 154 different haplotypes were identified, 127 (82.5%) of which were unique, with the most frequent haplotype occurring in 14 individuals (6.4%). The locus diversity ranged from 0.2050 at DYS392 to 0.8760 at DYS385. The haplotype diversity at the 17-loci resolution was calculated to be 0.9916, while the corresponding values for the extended (11 loci) and minimal (9 loci) haplotypes were estimated at 0.9735 and 0.9710, respectively. Comparison with 29 regional and global populations using correspondence analysis, neighbor joining (NJ) tree, and Rst genetic distance revealed that the Sousse population is highly diverse. This finding is consistent with historical data. Furthermore, the results of this study indicate a distinct genetic substructure among Tunisian populations. In conclusion, the present study demonstrated that the 17 Y-STRs analyzed are highly informative for individual identification, parentage analysis, and population genetic studies.
在本研究中，对来自突尼斯中东部苏塞的 218 名无关男性进行了 17 个 Y 染色体 STR（Y-STR）基因座的分型，以评估数据在法医学和群体遗传学方面的应用。共鉴定出 154 种不同的单倍型，其中 127 种（82.5%）为独特单倍型，最常见的单倍型出现在 14 个人中（6.4%）。17 个基因座的多样性范围从 DYS392 的 0.2050 到 DYS385 的 0.8760。17 个基因座分辨率下的单倍型多样性计算为 0.9916，而扩展（11 个基因座）和最小（9 个基因座）单倍型的相应值分别估计为 0.9735 和 0.9710。通过对应分析、邻接聚类（NJ）树和 Rst 遗传距离与 29 个区域和全球群体进行比较，结果表明苏塞人群具有高度多样性。这一发现与历史数据一致。此外，本研究的结果表明突尼斯人群之间存在明显的遗传亚结构。总之，本研究表明分析的 17 个 Y-STR 对个体识别、亲子关系分析和群体遗传学研究具有高度信息性。
