Department of Microbiology, PSGVP Mandal's ASC College Shahada, Maharashtra, 425 409 India.
Indian J Microbiol. 2009 Sep;49(3):230-2. doi: 10.1007/s12088-009-0036-7. Epub 2009 Jun 17.
We reported the optimum amount of PHB accumulated by Alcaligenes faecalis during its 24 h growth under nitrogen deficient conditions. After 24 h incubation decrease in the amount of PHB was recorded. Hypochlorite digestion of biomass of organism followed by extraction with a solvent system consisting of 1:1 mixture of ethanol and acetone resulted in efficient recovery of PHB vis-à-vis earlier methods. This solvent system gave a high recovery yield, i.e. 5.6 gL(-1) vis-à-vis earlier reported yield, 1.34 gL(-1) (by same method), 0.63 gL(-1) (by chloroform extraction method) and 1.1 gL(-1) (by dispersion method).
我们报道了在缺氮条件下，粪产碱杆菌在 24 小时生长过程中积累的最佳 PHB 量。经过 24 小时的孵育，记录到 PHB 的量减少。用次氯酸盐消化生物量，然后用由 1:1 混合的乙醇和丙酮组成的溶剂系统进行提取，与以前的方法相比，这导致了 PHB 的有效回收。与以前报道的产率相比，该溶剂系统的回收率较高，即 5.6 gL(-1)（相同方法为 1.34 gL(-1)），0.63 gL(-1)（氯仿提取法）和 1.1 gL(-1)（分散法）。
