Biotechnologie des Protéines Recombinantes à Visée Santé, University Bordeaux EA4135, F-33000, Bordeaux, France.
Microbiologyopen. 2012 Sep;1(3):326-39. doi: 10.1002/mbo3.33. Epub 2012 Sep 1.
Bacterial biofilms are complex cell communities found attached to surfaces and surrounded by an extracellular matrix composed of exopolysaccharides, DNA, and proteins. We investigated the whole-genome expression profile of Pseudomonas aeruginosa sessile cells (SCs) present in biofilms developed on a glass wool substratum. The transcriptome and proteome of SCs were compared with those of planktonic cell cultures. Principal component analysis revealed a biofilm-specific gene expression profile. Our study highlighted the overexpression of genes controlling the anthranilate degradation pathway in the SCs grown on glass wool for 24 h. In this condition, the metabolic pathway that uses anthranilate for Pseudomonas quinolone signal production was not activated, which suggested that anthranilate was primarily being consumed for energy metabolism. Transposon mutants defective for anthranilate degradation were analyzed in a simple assay of biofilm formation. The phenotypic analyses confirmed that P. aeruginosa biofilm formation partially depended on the activity of the anthranilate degradation pathway. This work points to a new feature concerning anthranilate metabolism in P. aeruginosa SCs.
细菌生物膜是附着在表面并被由胞外多糖、DNA 和蛋白质组成的细胞外基质包围的复杂细胞群落。我们研究了在玻璃纤维基底上形成的生物膜中存在的不动杆菌（Pseudomonas aeruginosa）定殖细胞（SC）的全基因组表达谱。将 SC 的转录组和蛋白质组与浮游细胞培养物进行了比较。主成分分析显示出生物膜特异性的基因表达谱。我们的研究强调了在玻璃纤维上生长 24 小时的 SC 中控制邻氨基苯甲酸降解途径的基因的过度表达。在这种条件下，用于假单胞菌喹诺酮信号产生的邻氨基苯甲酸代谢途径未被激活，这表明邻氨基苯甲酸主要被用于能量代谢。我们在简单的生物膜形成测定中分析了邻氨基苯甲酸降解缺陷的转座子突变体。表型分析证实，铜绿假单胞菌生物膜的形成部分取决于邻氨基苯甲酸降解途径的活性。这项工作指出了铜绿假单胞菌 SC 中邻氨基苯甲酸代谢的一个新特征。
