Department of Bioengineering, Tatung University, Taipei City, Taiwan.
BMC Complement Altern Med. 2012 Nov 23;12:226. doi: 10.1186/1472-6882-12-226.
Lonicera japonica Thunb. (Caprifoliaceae), a widely used traditional Chinese medicinal plant, is used to treat some infectious diseases and it may have uses as a healthy food and applications in cosmetics and as an ornamental groundcover. The ethanol extract of the flowering aerial parts of L. japonica (LJEE) was investigated for its healing efficiency in a rat excision wound model.
Excision wounds were inflicted upon three groups of eight rats each. Healing was assessed by the rate of wound contraction in skin wound sites in rats treated with simple ointment base, 10% (w/w) LJEE ointment, or the reference standard drug, 0.2% (w/w) nitrofurazone ointment. The effects of LJEE on the contents of hydroxyproline and hexosamine during healing were estimated. The antimicrobial activity of LJEE against microorganisms was also assessed. The in vivo anti-inflammatory activity of LJEE was investigated to understand the mechanism of wound healing.
LJEE exhibited significant antimicrobial activity against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Candida albicans, and Candida tropicalis. The ointment formulation prepared with 10% (w/w) LJEE exhibited potent wound healing capacity as evidenced by the wound contraction in the excision wound model. The contents of hydroxyproline and hexosamine also correlated with the observed healing pattern. These findings were supported by the histopathological characteristics of healed wound sections, as greater tissue regeneration, more fibroblasts, and angiogenesis were observed in the 10% (w/w) LJEE ointment-treated group. The results also indicated that LJEE possesses potent anti-inflammatory activity, as it enhanced the production of anti-inflammatory cytokines that suppress proinflammatory cytokine production.
The results suggest that the antimicrobial and anti-inflammatory activities of LJEE act synergistically to accelerate wound repair.
忍冬（忍冬科）是一种广泛使用的传统中药，用于治疗一些传染病，也可用作健康食品、化妆品以及观赏地被植物。本研究考察了忍冬花的醇提物（LJEE）在大鼠切口模型中的愈合效率。
将三组 8 只大鼠的皮肤切口造模。通过比较单纯软膏基质组、10%（w/w）LJEE 软膏组和 0.2%（w/w）呋喃西林软膏（参比标准药物）对大鼠皮肤伤口收缩率，评估 LJEE 的愈合效果。还评估了 LJEE 对愈合过程中羟脯氨酸和己糖胺含量的影响。此外，还评估了 LJEE 对微生物的抗菌活性。通过体内抗炎活性研究，以了解其促进伤口愈合的机制。
LJEE 对金黄色葡萄球菌、表皮葡萄球菌、大肠杆菌、白色念珠菌和热带念珠菌均表现出较强的抗菌活性。含有 10%（w/w）LJEE 的软膏制剂表现出较强的促愈合能力，这可从切口模型中的伤口收缩得到证明。羟脯氨酸和己糖胺的含量也与观察到的愈合模式相关。组织学特征表明，LJEE 可促进组织再生，增加成纤维细胞和血管生成，这一结果支持了上述发现。此外，LJEE 还具有较强的抗炎活性，可促进抗炎细胞因子的产生，抑制促炎细胞因子的产生。
研究结果表明，LJEE 的抗菌和抗炎活性协同作用，可加速伤口修复。
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