Department of Dermatology, E-Da Hospital, I-Shou University, Kaohsiung City 84001, Taiwan.
School of Chinese Medicine for Post Baccalaureate, College of Medicine, I-Shou University, Kaohsiung City 84001, Taiwan.
Nutrients. 2024 Sep 19;16(18):3161. doi: 10.3390/nu16183161.
Hispidin, a polyphenol component mainly derived from the medicinal mushroom species and , shows promise for biomedical applications, yet its potential in wound healing remains largely unexplored. This research investigates the wound healing effects of hispidin through in vitro and in vivo experiments, while also evaluating its antimicrobial properties and safety profile.
In vitro scratch assays were conducted to evaluate the impact of hispidin on the migration of NIH-3T3 cells. The wound healing potential of hispidin was assessed in rats using excision wounds, dead space wounds, and linear incisions, treated with various topical ointments including a simple ointment, 2.5% (/) and a 5% (/) hispidin ointment, and a 0.2% (/) nitrofurazone ointment, administered at 0.2 g daily for 14 days.
Hispidin demonstrated antimicrobial properties and was particularly effective against . Hispidin enhanced NIH-3T3 cell viability, and promoted wound closure in scratch assays, correlating with increased levels of FGF21, TGF-β1, EGF, and VEGF. In excision wound models, the 5% (/) hispidin ointment improved wound contraction, epithelialization, tissue regeneration, fibroblast activity, and angiogenesis. In the granulation tissue from dead space wound models, hispidin reduced pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) and lipid peroxidation, while increasing anti-inflammatory cytokines (IL-10) and antioxidant activities (SOD, GPx, CAT), along with connective tissue markers like hydroxyproline, hexosamine, and hexuronic acid. Hispidin also enhanced wound breaking strength in incision models. Acute dermal toxicity studies indicated no adverse effects at 2000 mg/kg.
These findings highlight hispidin's potential in wound care, demonstrating its antimicrobial, regenerative, and safety properties.
棘白菌素是一种多酚类成分，主要来源于药用蘑菇 和 ，具有生物医学应用的潜力，但在伤口愈合方面的潜力尚未得到充分探索。本研究通过体外和体内实验研究了棘白菌素的伤口愈合作用，同时评估了其抗菌性能和安全性。
通过体外划痕实验评估棘白菌素对 NIH-3T3 细胞迁移的影响。在大鼠中使用切除伤口、死腔伤口和线性切口评估棘白菌素的伤口愈合潜力，用各种局部软膏治疗，包括简单软膏、2.5%（/）和 5%（/）棘白菌素软膏以及 0.2%（/）呋喃西林软膏，每天 0.2 g，持续 14 天。
棘白菌素具有抗菌性能，对 尤为有效。棘白菌素增强了 NIH-3T3 细胞的活力，并在划痕实验中促进了伤口闭合，这与 FGF21、TGF-β1、EGF 和 VEGF 水平的升高相关。在切除伤口模型中，5%（/）棘白菌素软膏改善了伤口收缩、上皮化、组织再生、成纤维细胞活性和血管生成。在死腔伤口模型的肉芽组织中，棘白菌素减少了促炎细胞因子（TNF-α、IL-6、IL-1β）和脂质过氧化，同时增加了抗炎细胞因子（IL-10）和抗氧化活性（SOD、GPx、CAT），以及羟脯氨酸、己糖胺和己糖醛酸等结缔组织标志物。棘白菌素还增强了切口模型中的伤口断裂强度。急性皮肤毒性研究表明，在 2000mg/kg 时没有不良反应。
这些发现强调了棘白菌素在伤口护理方面的潜力，展示了其抗菌、再生和安全性特性。
