Woerly S, Marchand R, Lavallée C
Laboratoire de Neurobiologie, Hôpital de l'Enfant-Jésus et Université Laval, Québec, Canada.
Biomaterials. 1990 Mar;11(2):97-107. doi: 10.1016/0142-9612(90)90123-8.
Various poly (2-hydroxyethyl methacrylate)-collagen and poly (glyceryl methacrylate)-collagen composite hydrogels with varying porosities and cross-linking densities were implanted into the cortex of adult rat brains to provide mechanical guiding substrates for wound healing and tissue ingrowth. The hydrogels were well tolerated by the neural tissue. After 2 and 3 month, hyper- and macroporous hydrogels (poly(glyceryl methacrylate)) with interconnected channel systems were penetrated by neural tissue and elements of extracellular matrices, with differences in the degree and the topographic patterning of tissue ingrowth according to the type of samples. These differences were ascribed to the geometry, size of the pore interconnections and the mechanical properties of the polymers. Hyper- and microporous hydrogels (poly(2-hydroxyethyl methacrylate)) and hydrogels without collagen were not penetrated by the host tissue. The compatibility of the polymers with the neural tissue was also tested in vitro. This study suggests a new approach to repair brain lesions consisting of loss of tissue volume.
将具有不同孔隙率和交联密度的各种聚（甲基丙烯酸2-羟乙酯）-胶原蛋白和聚（甲基丙烯酸甘油酯）-胶原蛋白复合水凝胶植入成年大鼠大脑皮层，以提供用于伤口愈合和组织向内生长的机械引导基质。神经组织对这些水凝胶耐受性良好。2个月和3个月后，具有相互连接通道系统的超多孔和大孔水凝胶（聚（甲基丙烯酸甘油酯））被神经组织和细胞外基质成分穿透，根据样品类型，组织向内生长的程度和地形模式存在差异。这些差异归因于聚合物的几何形状、孔互连尺寸和机械性能。超微孔和微孔水凝胶（聚（甲基丙烯酸2-羟乙酯））以及不含胶原蛋白的水凝胶未被宿主组织穿透。还在体外测试了聚合物与神经组织的相容性。这项研究提出了一种修复由组织体积丧失引起的脑损伤的新方法。
