de Lima Marques Joyce Figueiredo, Aguilar Milagros Falcon, Vieira-Junior Waldemir Francisco, Pecorari Vanessa Gallego Arias, Rischka Klaus, Aguiar Flávio Henrique Baggio
Department of Restorative Dentistry, Piracicaba School of Dentistry, University of Campinas, Av. Limeira, 901, Piracicaba, SP, CEP: 13414-903, Brazil.
Department of Children's Health Sciences and Dentistry, - Area of Biostatistics, Piracicaba School of Dentistry, University of Campinas, Av. Limeira, 901, Piracicaba, SP, CEP: 13414-903, Brazil.
Clin Oral Investig. 2025 Mar 19;29(4):193. doi: 10.1007/s00784-025-06276-7.
This study aimed to evaluate the effects on morphology and permeability of dentin after an erosive and abrasive cycling protocol followed by treatment with an experimental solution containing dopamine.
Dentin hypersensitivity was simulated in human dentin discs by applying EDTA for 5 min. After assessing maximum dentin permeability (initial), specimens were randomly allocated into four groups: distilled water (control), NaF 0.05% solution, a commercial solution (Colgate Sensitive Pró-AlívioTM), or an experimental solution containing dopamine and laccase (n = 15). An erosive/abrasive cycling protocol was applied, including 0.3% citric acid for 2 min 4x/day, brushing for 5 s 2x/day, treatments for 5 min 2x/day. Dentin permeability was reassessed (final). Percentage permeability for each time point was calculated relative to maximum permeability (%Lp) and analyzed by generalized linear mixed models (α = 0.05). Surface effects were analyzed by scanning electron microscopy.
A significant reduction in permeability occurred in all groups post-cycling and treatment (p < 0.05), with no significant differences between groups (p = 0.6082). All groups exhibited sparse surface deposits, with few occluded tubules. Tubules appeared smaller in diameter in the Colgate and experimental solution groups, while distilled water and NaF groups showed more open tubules.
In this model, dopamine demonstrated a limited effect, showing no significant protection of the dentin surface or reduction in permeability, similar to the other tested solutions.
Changes in the habits of modern society have increased the prevalence of tooth wear and dentin hypersensitivity. In this context, it is essential to develop not only new active ingredients to prevent or minimize mineral loss but also new treatment methods.
本研究旨在评估在进行侵蚀性和磨耗性循环方案后，用含多巴胺的实验溶液处理对牙本质形态和渗透性的影响。
通过应用乙二胺四乙酸（EDTA）5分钟，在人牙本质盘中模拟牙本质过敏。在评估最大牙本质渗透性（初始值）后，将标本随机分为四组：蒸馏水（对照组）、0.05%氟化钠溶液、一种市售溶液（高露洁敏感专业舒缓牙膏）或含多巴胺和漆酶的实验溶液（n = 15）。应用侵蚀性/磨耗性循环方案，包括每天4次、每次2分钟的0.3%柠檬酸处理，每天2次、每次5秒的刷牙处理，每天2次、每次5分钟的处理。重新评估牙本质渗透性（最终值）。计算每个时间点相对于最大渗透性的渗透率百分比（%Lp），并通过广义线性混合模型进行分析（α = 0.05）。通过扫描电子显微镜分析表面效应。
在循环和处理后，所有组的渗透性均显著降低（p < 0.05），组间无显著差异（p = 0.6082）。所有组均表现出稀疏的表面沉积物，封闭的小管较少。高露洁组和实验溶液组的小管直径似乎较小，而蒸馏水组和氟化钠组的小管开口较多。
在该模型中，多巴胺显示出有限的作用，与其他测试溶液相似，对牙本质表面没有显著的保护作用，也没有降低渗透性。
现代社会生活习惯的改变增加了牙齿磨损和牙本质过敏的患病率。在此背景下，不仅要开发新的活性成分以预防或减少矿物质流失，还要开发新的治疗方法，这一点至关重要。
