紫外线照射的人成纤维细胞中嘧啶二聚体与复制DNA之间的关系。
The relationship between pyrimidine dimers and replicating DNA in UV-irradiated human fibroblasts.
作者信息
Lehmann A R
出版信息
Nucleic Acids Res. 1979 Dec 11;7(7):1901-12. doi: 10.1093/nar/7.7.1901.
Abstract
The relationship between pyrimidine dimers (measured as endonuclease-sensitive sites) and newly-synthesized DNA has been examined in several different ways, with the following results:- 1. After UV-irradiation of normal human fibroblasts the frequency of pyrimidine dimer sites in sections of DNA which have been synthesized immediately before the UV-irradiation is similar to that in the bulk DNA. 2. The frequency of pyrimidine dimer sites in the parental strands of replicating DNA in UV-irradiated normal human fibroblasts is similar to that in the bulk DNA. 3. In UV-irradiated XP variant cells the size of DNA synthesized in the presence of caffeine immediately after UV irradiation accurately corresponds with the average interdimer distance in the parental DNA. This suggests that in this experimental situation each pyrimidine dimer gives rise to a disocntinuity or a termination site in the daughter strand.
摘要
嘧啶二聚体（以内切核酸酶敏感位点衡量）与新合成DNA之间的关系已通过几种不同方式进行了研究，结果如下：- 1. 正常人类成纤维细胞经紫外线照射后，在紫外线照射前立即合成的DNA片段中，嘧啶二聚体位点的频率与总体DNA中的频率相似。2. 紫外线照射的正常人类成纤维细胞中复制DNA的亲代链中嘧啶二聚体位点的频率与总体DNA中的频率相似。3. 在紫外线照射的着色性干皮病变异细胞中，紫外线照射后立即在咖啡因存在下合成的DNA大小与亲代DNA中平均二聚体间距精确对应。这表明在这种实验情况下，每个嘧啶二聚体在子代链中产生一个间断或一个终止位点。
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