二聚体对紫外线照射的中国仓鼠卵巢细胞中新合成DNA大小的意义。
Significance of dimers to the size of newly synthesized DNA in UV-irradiated Chinese hamster ovary cells.
作者信息
Clarkson J M, Hewitt R R
出版信息
Biophys J. 1976 Oct;16(10):1155-64. doi: 10.1016/S0006-3495(76)85764-5.
Abstract
DNA synthesized after UV irradiation is smaller than that in unirradiated cells even when pulse-labeling times are increased to compensate for the overall reduction in the rate of DNA replication. By isolating newly replicated DNA, incubating it with dimer-specific endonuclease from Micrococcus luteus, and analyzing it on alkaline sucrose gradients, we have been able to demonstrate that this DNA is synthesized in segments corresponding in size to the interdimer distance on the parental strand. In addition, the same DNA analyzed on neutral gradients shows no reduction in molecular weight as a result of UV irradiation and/or endonuclease digestion. Our data are thus inconsistent with the presence of "gaps" in newly synthesized DNA opposite the dimers on the parental strand. We suggest that if such gaps are produced as a result of delayed synthesis around dimers, they are filled before the growing point reaches the next dimer.
摘要
即使将脉冲标记时间延长以补偿DNA复制速率的整体降低，紫外线照射后合成的DNA仍比未照射细胞中的DNA小。通过分离新复制的DNA，将其与来自藤黄微球菌的二聚体特异性内切酶一起孵育，并在碱性蔗糖梯度上进行分析，我们能够证明这种DNA是以与亲本链上二聚体间距离大小相对应的片段形式合成的。此外，在中性梯度上分析相同的DNA时，未发现紫外线照射和/或内切酶消化导致其分子量降低。因此，我们的数据与亲本链上与二聚体相对的新合成DNA中存在“缺口”的情况不一致。我们认为，如果由于二聚体周围合成延迟而产生这种缺口，那么在生长点到达下一个二聚体之前，这些缺口就会被填补。
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本文引用的文献
1
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2
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3
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4
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6
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