College of Food Science and Engineering, Northwest A&F University, 712100 Yangling, PR China.
College of Food Science and Engineering, Northwest A&F University, 712100 Yangling, PR China.
Food Chem. 2019 Feb 15;274:494-499. doi: 10.1016/j.foodchem.2018.09.037. Epub 2018 Sep 5.
A novel acidic polysaccharide fraction (PZMP2-2) was isolated from Ziziphus Jujuba cv. Muzao fruit via hot water extraction and purified by DEAE-Sepharose FF and Sephacryl S-300 column chromatography. PZMP2-2 consisted of rhamnose, arabinose, xylose, galactose, and galacturonic acid in a ratio of 1.18: 5.23: 0.22: 2.68: 2.20, with a molecular weight of 62.73 kDa. Structural features of PZMP2-2 were investigated using FT-IR, SEM, AFM, GC-MS analysis and NMR spectroscopy. Methylation and GC-MS analysis revealed that the main glycosidic bonds in PZMP2-2 comprised 1-linked Galp, 1,3-linked Araf, 1,2,4-linked Rhap, 1,3-linked Galp, 1,4-linked GalpA and 1,3,5-linked Araf. NMR analysis indicate that PZMP 2-2 has a linear backbone of (1 → 4)-linked GlcpA and (1 → 2,4)-linked Rhap residues, with branches at the O-4 position, consisting of Araf and Galp residues.
一种新型酸性多糖组分（PZMP2-2）从大枣（Ziziphus Jujuba cv. Muzao）果实中通过热水提取，并用 DEAE-Sepharose FF 和 Sephacryl S-300 柱层析进行纯化分离得到。PZMP2-2 由鼠李糖、阿拉伯糖、木糖、半乳糖和半乳糖醛酸以 1.18:5.23:0.22:2.68:2.20 的比例组成，分子量为 62.73 kDa。PZMP2-2 的结构特征通过傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）、原子力显微镜（AFM）、气相色谱-质谱联用分析（GC-MS 分析）和核磁共振波谱（NMR 光谱）进行研究。甲基化和 GC-MS 分析表明，PZMP2-2 中的主要糖苷键包括 1-连接的半乳糖（Galp）、1,3-连接的阿拉伯糖（Araf）、1,2,4-连接的鼠李糖（Rhap）、1,3-连接的半乳糖（Galp）、1,4-连接的半乳糖醛酸（GalpA）和 1,3,5-连接的阿拉伯糖（Araf）。NMR 分析表明，PZMP2-2 具有（1→4）-连接的葡萄糖醛酸（GlcpA）和（1→2,4）-连接的鼠李糖（Rhap）残基的线性主链，在 O-4 位置有支链，由阿拉伯糖和半乳糖残基组成。
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