Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.
Adv Healthc Mater. 2013 Jan;2(1):186-94. doi: 10.1002/adhm.201200159. Epub 2012 Nov 19.
Scaffolds for tissue engineering are usually designed to support cell viability with large adhesion surfaces and high permeability to nutrients and oxygen. Recent experiments support the idea that, in addition to surface roughness, elasticity and chemistry, the macroscopic geometry of the substrate also contributes to control the kinetics of tissue deposition. In this study, a previously proposed model for the behavior of osteoblasts on curved surfaces is used to predict the growth of bone matrix tissue in pores of different shapes. These predictions are compared to in vitro experiments with MC3T3-E1 pre-osteoblast cells cultivated in two-millimeter thick hydroxyapatite plates containing prismatic pores with square- or cross-shaped sections. The amount and shape of the tissue formed in the pores measured by phase contrast microscopy confirms the predictions of the model. In cross-shaped pores, the initial overall tissue deposition is twice as fast as in square-shaped pores. These results suggest that the optimization of pore shapes may improve the speed of ingrowth of bone tissue into porous scaffolds.
用于组织工程的支架通常设计为具有大的粘附表面和高的营养物质和氧气通透性，以支持细胞活力。最近的实验支持这样的观点，即除了表面粗糙度、弹性和化学性质外，基底的宏观几何形状也有助于控制组织沉积的动力学。在这项研究中，使用先前提出的关于成骨细胞在曲面上行为的模型来预测不同形状的孔中骨基质组织的生长。将这些预测与在含有棱柱形孔的 2 毫米厚羟磷灰石板中培养的 MC3T3-E1 前成骨细胞的体外实验进行比较，所述孔具有正方形或十字形截面。相差显微镜测量的孔中形成的组织的量和形状证实了模型的预测。在十字形孔中，初始的整体组织沉积速度是方形孔的两倍。这些结果表明，优化孔的形状可以提高骨组织向多孔支架中生长的速度。
