Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
Am J Trop Med Hyg. 2013 Feb;88(2):260-6. doi: 10.4269/ajtmh.2012.12-0477. Epub 2012 Nov 26.
Epidemic dengue has emerged throughout the tropical world. In the continued absence of a vaccine against dengue virus (DENV), mosquito vector surveillance and control programs are essential to reduce human infections. An effective test to detect DENV in infected mosquitoes would be a valuable addition to the surveillance effort. We investigated DENV detection in infected Aedes aegypti using a commercially available DENV non-structural protein 1 (NS1) ELISA kit (Platelia Dengue NS1 Ag), and by reverse transcription-polymerase chain reaction (RT-PCR) and virus isolation assays. The DENV-infected mosquitoes were subjected to field-relevant conditions and assayed individually and pooled with uninfected mosquitoes. Overall, DENV NS1 antigen was detected in 98% of infected mosquitoes/pools versus 79% for RT-PCR and 29% for virus isolation. Our results indicate that NS1 is an excellent analyte for detection of DENV in Ae. aegypti and that the tested NS1 antigen kit provides a sensitive, rapid, and convenient test for DENV surveillance in mosquitoes.
登革热疫情已在整个热带地区出现。在仍没有针对登革病毒 (DENV) 的疫苗的情况下，蚊子媒介监测和控制计划对于减少人类感染至关重要。一种能够有效检测感染蚊子中 DENV 的检测方法将是监测工作的有力补充。我们使用市售的登革热非结构蛋白 1（NS1）ELISA 试剂盒（Platelia Dengue NS1 Ag）和逆转录-聚合酶链反应（RT-PCR）及病毒分离检测法，研究了在感染的埃及伊蚊中检测 DENV 的方法。将受感染的蚊子置于与现场相关的条件下进行检测，并分别对其进行检测和与未感染的蚊子混合检测。结果显示，在感染的蚊子/蚊子群中，DENV NS1 抗原的检出率为 98%，而 RT-PCR 为 79%，病毒分离法为 29%。我们的研究结果表明，NS1 是检测埃及伊蚊中 DENV 的理想分析物，而经过测试的 NS1 抗原试剂盒则为蚊子中的 DENV 监测提供了一种灵敏、快速和便捷的检测方法。
