非洲传统草药中的促有丝分裂活性（第二部分）。

Mitogenic activities in African traditional herbal medicines (Part II).

机构信息

Kobe Pharmaceutical University, 4-19-1, Motoyamakita-machi, Higashinadaku, Kobe 658, Japan.

出版信息

Phytomedicine. 1996 Mar;2(4):335-9. doi: 10.1016/S0944-7113(96)80078-X.

PMID:23194771

Abstract

Mitogenic activities in African traditional herbal medicines were examined on human peripheral blood lymphocytes and mouse spleen cells using protein fractions obtained from their extracts by precipitation with ammonium sulfate. Target specificity for these mitogens was investigated by using isolated T cells and lymphocytes from athymic nude mice. Among 20 plants investigated, potent mitogenic activities for both human and mouse lymphocytes were found in 7 plants: Monanthotaxis sp. (Annonaceae), Uvaria lucida (Annonaceae), Maytenus buchananii (Celastraceae), Lonchocarpus bussei (Leguminosae), Phytolacca dodecandra (Phytolaccaceae), Phytolacca octandra (Phytolaccaceae), and Toddalia asiatica (Rutaceae). The U. lucida stem demonstrated the highest activity among all and induced mitogenesis both in human and mouse isolated T cells, but not in lymphocytes from athymic nude mice.

摘要

采用硫酸铵沉淀法从非洲传统草药提取物中获得蛋白质级分，研究了它们对人外周血淋巴细胞和小鼠脾细胞的有丝分裂活性。通过使用分离的 T 细胞和无胸腺裸鼠淋巴细胞，研究了这些有丝分裂原的靶特异性。在所研究的 20 种植物中，有 7 种植物对人和小鼠淋巴细胞具有强烈的有丝分裂活性：Monanthotaxis sp.（番荔枝科）、Uvaria lucida（番荔枝科）、Maytenus buchananii（卫矛科）、Lonchocarpus bussei（豆科）、Phytolacca dodecandra（商陆科）、Phytolacca octandra（商陆科）和 Toddalia asiatica（芸香科）。其中，乌药茎的活性最高，不仅能诱导人和小鼠分离的 T 细胞有丝分裂，也能诱导无胸腺裸鼠淋巴细胞有丝分裂。