Tachibana Y, Kato A, Nishiyama Y, Kawanishi K, Tobe H, Juma F D, Ogeto J O, Mathenge S G
Kobe Women's College of Pharmacy, Japan.
Planta Med. 1993 Aug;59(4):354-8. doi: 10.1055/s-2006-959700.
Mitogenic activities in African traditional herbal medicines were examined using protein fractions obtained from their extracts by precipitation with ammonium sulfate. Potent mitogenic activities for human and mouse lymphocytes were found in the three plants: Croton macrostachyus, Croton megalocarpus (Euphorbiaceae), and Phytolacca dodecandra (Phytolaccaceae). All the gel chromatographic patterns of these protein fractions progressed toward the smaller molecule site with pronase treatment, while their mitogenic activities decreased significantly. Protein fractions from these three plants induced mitogenesis both in human and mouse isolated T cells, but not in lymphocytes from athymic nude mice. By testing further fractionated protein fractions with gel filtration chromatography, it was found that all three plants contained several mitogens having different molecule sizes.
利用通过硫酸铵沉淀从非洲传统草药提取物中获得的蛋白质组分，检测了其促有丝分裂活性。在三种植物中发现了对人和小鼠淋巴细胞有强效促有丝分裂活性：大戟科大戟属的大戟和巨果巴豆，以及商陆科商陆属的商陆。这些蛋白质组分的所有凝胶色谱图谱经链霉蛋白酶处理后都向小分子位点移动，而它们的促有丝分裂活性则显著降低。这三种植物的蛋白质组分在人和小鼠分离的T细胞中均可诱导有丝分裂，但在无胸腺裸鼠的淋巴细胞中则不能。通过用凝胶过滤色谱法进一步检测分级分离的蛋白质组分，发现这三种植物都含有几种分子大小不同的促有丝分裂原。
