Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, PR China.
Clin Biochem. 2013 Mar;46(4-5):346-53. doi: 10.1016/j.clinbiochem.2012.11.012. Epub 2012 Nov 27.
To evaluate a method for predicting renal function recoverability after the relief of obstructive uropathy using (1)H NMR-based metabonomics.
This study included 16 bilateral ureteral obstruction (BUO) patients and 9 unilateral ureteral obstruction (UUO) patients. The obstructions in all of the patients were successfully relieved after treatment. Urine samples at different time points before and after treatment were obtained, and their (1)H NMR spectra were recorded and analyzed using multivariate statistical techniques combined with quantitative statistical analyses.
A distinctive pattern in the metabolite profile of urine samples from preoperation and early and late stages after treatment was observed, which was in good agreement with the clinical chemistry analyses. Some important systemic perturbations in endogenous metabolites were correlated with different recoverability stages of renal function after obstruction relief. In addition, the OPLS-DA model of BUO patients could help predict the recovery of renal function of UUO patients.
The results suggest that (1)H NMR-based metabonomics can be used to evaluate the recoverability of renal function after obstruction relief. Our results suggest that (1)H NMR-based metabonomics may help stratify patients according to disease stages, provide prognostic information, and be useful for postoperative surveillance.
利用（1）H 核磁共振代谢组学评估梗阻性尿路后肾功能恢复能力的预测方法。
本研究纳入 16 例双侧输尿管梗阻（BUO）患者和 9 例单侧输尿管梗阻（UUO）患者。所有患者的梗阻在治疗后均成功缓解。在治疗前后不同时间点采集尿液样本，并用多变量统计技术结合定量统计分析对其（1）H NMR 图谱进行记录和分析。
观察到术前和治疗后早期及晚期尿液样本代谢谱的特征模式，与临床化学分析一致。一些与梗阻缓解后肾功能不同恢复阶段相关的内源性代谢物的重要系统扰动。此外，BUO 患者的 OPLS-DA 模型可帮助预测 UUO 患者肾功能的恢复。
结果表明，（1）H 核磁共振代谢组学可用于评估梗阻缓解后肾功能的恢复能力。我们的结果表明，（1）H 核磁共振代谢组学可能有助于根据疾病阶段对患者进行分层，提供预后信息，并有助于术后监测。
