Department of Microbiology and Immunology, Faculty of Cs. Ex. Fco-Qcas y Naturales, University of Río Cuarto, Río Cuarto, Córdoba, Argentina.
J Dairy Res. 2013 Feb;80(1):28-35. doi: 10.1017/S0022029912000581. Epub 2012 Nov 9.
The effect of intramammary inoculation of Lactobacillus perolens CRL 1724 on bovine udders at drying off was evaluated through histological examination of the canal and cistern tissues. The persistence of the strain in the udder 7 d post inoculation was also determined. Lb. perolens CRL 1724 was recovered from all mammary quarters and no clinical signs or teat damage were observed after inoculation of 10(6) cfu/ml. The udders showed a normal structural aspect and there were no modifications of the milk appearance. Lb. perolens CRL 1724 cells were evidenced on the surface of the epithelial cells of the cistern without causing any morphological modifications or cell alterations. Lb. perolens CRL 1724 produces a mild inflammatory reaction, characterized by recruitment of neutrophils to the epithelial zone and a slight hyperaemia into blood vessels. This preliminary study provides important information for further studies directed towards the inclusion of Lb. perolens CRL 1724 in the design of probiotic products for preventing bovine mastitis in non-lactating dairy cows.
通过对乳道和乳池组织的组织学检查，评估了在干奶期经乳内接种植物乳杆菌 CRL 1724 对奶牛乳房的影响。还确定了该菌株在接种后 7 天内是否在乳房中持续存在。从所有乳房 quarters 中都回收了植物乳杆菌 CRL 1724，并且在接种 10(6)cfu/ml 后没有观察到临床症状或乳头损伤。乳房表现出正常的结构外观，并且牛奶外观没有任何变化。植物乳杆菌 CRL 1724 细胞在乳池的上皮细胞表面被证实，而不会引起任何形态改变或细胞改变。植物乳杆菌 CRL 1724 产生轻度炎症反应，其特征是招募中性粒细胞到上皮区，并使血管轻微充血。这项初步研究为进一步研究提供了重要信息，目的是将植物乳杆菌 CRL 1724 纳入预防非泌乳奶牛乳腺炎的益生菌产品的设计中。
