基于重组嗜热毁丝霉纤维二糖脱氢酶的纤维素氧化和漂白工艺。
Cellulose oxidation and bleaching processes based on recombinant Myriococcum thermophilum cellobiose dehydrogenase.
机构信息
Graz University of Technology, Institute of Environmental Biotechnology, Petersgasse 12/1, A-8010 Graz, Austria.
出版信息
Enzyme Microb Technol. 2013 Jan 10;52(1):60-7. doi: 10.1016/j.enzmictec.2012.10.007. Epub 2012 Oct 29.
Myriococcum thermophilum cellobiose dehydrogenase (MtCDH) was expressed in Pichia pastoris using the pPICZαA expression vector under the control of methanol inducible AOX promoter. The purified recombinant MtCDH with a specific activity of 3.1 Umg(-1) was characterized to obtain kinetic constants for various carbohydrate substrates. Additionally, the C1 oxidation of the reducing ends of cellobiose, cellotetraose and maltotriose by MtCDH was verified by HPLC-MS. MtCDH was employed to oxidize several different cellulose-based materials by production of hydrogen peroxide. Based on the obtained results a one-pot enzymatic scouring/bleaching process for cotton fabrics was developed using pectinases as scouring agent and MtCDH to produce H(2)O(2) for bleaching. An average increase in whiteness (Berger) ΔE of 26 and an average 95% increase in wettability were observed in all MtCDH treated fabrics. In addition, MtCDH oxidized typical colored cotton flavonoids (morin, rutin, isoquercitrin).
嗜热毁丝霉木聚糖酶 (MtCDH) 在毕赤酵母中表达，使用 pPICZαA 表达载体，在甲醇诱导的 AOX 启动子的控制下。纯化的重组 MtCDH 比活性为 3.1 Umg(-1)，对各种碳水化合物底物的动力学常数进行了表征。此外，通过 HPLC-MS 验证了 MtCDH 对纤维二糖、纤维四糖和麦芽三糖还原端的 C1 氧化。MtCDH 用于通过产生过氧化氢氧化几种不同的基于纤维素的材料。根据获得的结果，使用果胶酶作为精炼剂，用 MtCDH 生产 H(2)O(2)进行漂白，开发了一种用于棉织物的一锅式酶精炼/漂白工艺。所有经 MtCDH 处理的织物的白度（Berger）ΔE 平均增加 26，润湿性平均增加 95%。此外，MtCDH 氧化了典型的有色棉类黄酮（桑色素、芦丁、异槲皮苷）。
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