Jiangsu Key laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, PR China.
Colloids Surf B Biointerfaces. 2013 Mar 1;103:52-8. doi: 10.1016/j.colsurfb.2012.10.025. Epub 2012 Oct 29.
Grafting-from has proven to be a very effective way to create high grafting densities and well-controlled polymer chains on different kinds of surfaces. In this work, we aim to graft zwitterionic brush from cellulose membrane (CM) via ARGET-ATRP (Activator Regenerated by Electron Transfer ATRP) method indirectly for blood compatibility improvement. Characterization of the CM substrates before and after modification was carried out by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), water contact angle measurements, X-ray photoelectron spectroscopy analysis, and atomic force microscopy, respectively. The results demonstrated zwitterionic brushes were successfully grafted on the CM surfaces, and the content of the grafted layer increased gradually with the polymerization time. The platelet adhesion, hemolytic test and plasma protein adsorption results indicated the cellulose membrane had significantly excellent blood compatibility featured on lower platelet adhesion and protein adsorption without causing hemolysis. The functionalized cellulose substrate could have a great potential usage for biomedical applications.
接枝法已被证明是一种非常有效的方法，可以在不同类型的表面上形成高接枝密度和可控的聚合物链。在这项工作中，我们旨在通过ARGET-ATRP（电子转移 ATRP 的引发剂再生）方法将两性离子刷从纤维素膜（CM）间接接枝，以提高血液相容性。通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）、水接触角测量、X 射线光电子能谱分析和原子力显微镜分别对修饰前后的 CM 基底进行了表征。结果表明，两性离子刷成功地接枝到 CM 表面上，并且接枝层的含量随着聚合时间的增加而逐渐增加。血小板黏附、溶血试验和血浆蛋白吸附结果表明，纤维素膜具有优异的血液相容性，血小板黏附率和蛋白吸附率低，且不会引起溶血。功能化纤维素基底在生物医学应用中具有很大的潜在用途。
