Medical Entomology Department, School of Public Health, Urmia University of Medical Sciences, Urmia, Iran.
Acta Trop. 2013 Apr;126(1):63-74. doi: 10.1016/j.actatropica.2012.11.003. Epub 2012 Dec 1.
Molecular studies on population genetics of speciation across Iran have recently started. Morphological and molecular studies have showed that 25 species of genus Anopheles are present in the country; however, relationships between vector and non-vector species as well as compatibility of morphological characters with molecular data have not been verified. Molecular phylogenetic analysis was undertaken on the Anopheles and Cellia subgenus members internal transcribed spacer 2 (ITS2) sequences submitted to GenBank among the Oriental and Palearctic members in north and southern Iran. rDNA-ITS2 sequences were extracted from the GenBank and analyzed using bioinformatics softwares: BLAST, ITS2 annotation tool (version 3.0.13), ClustalW, and MEGA5 in neighbor-joining and maximum likelihood algorithms. There are not any submitted sequences in GenBank from Iran for the following seven species: Anopheles algeriensis, Anopheles marteri, Anopheles plumbeus, Anopheles peditaeniatus, Anopheles melanoon, Anopheles subpictus, and Anopheles mongolensis; therefore, they have not been included in the study. Although these molecular-based phylogenetic trees match well enough with classical morphological taxonomy, the arrangement of species did not match with morphological classification in some cases. Correct species identification is essential for control of vector born disease such as malaria; therefore, phylogenetic methods will help to understand the relationship among the members of the target species within the genus Anopheles. It could also help us to design molecular markers for species differentiation particularly in cryptic species, which is difficult to classify them based on morphological features.
近年来，针对伊朗物种形成的种群遗传学的分子研究已经开始。形态学和分子研究表明，该国存在 25 种疟蚊属物种；然而，尚未验证媒介种和非媒介种之间的关系以及形态特征与分子数据的兼容性。在伊朗北部和南部的东方和古北部分子成员的内转录间隔区 2（ITS2）序列提交给 GenBank 后，对疟蚊和 Cellia 亚属成员进行了分子系统发育分析。rDNA-ITS2 序列从 GenBank 中提取，并使用生物信息学软件进行分析：BLAST、ITS2 注释工具（版本 3.0.13）、ClustalW 和 MEGA5 在邻接法和最大似然算法中。以下七个物种在 GenBank 中没有提交序列：Anopheles algeriensis、Anopheles marteri、Anopheles plumbeus、Anopheles peditaeniatus、Anopheles melanoon、Anopheles subpictus 和 Anopheles mongolensis；因此，它们未包含在研究中。尽管这些基于分子的系统发育树与经典形态分类学足够匹配，但在某些情况下，物种的排列与形态分类不匹配。正确的物种鉴定对于控制疟疾等媒介传播疾病至关重要；因此，系统发育方法将有助于了解疟蚊属目标物种成员之间的关系。它还可以帮助我们设计用于物种分化的分子标记，特别是在难以根据形态特征对其进行分类的隐种中。
