伊朗实验室条件下长跗库蚊（蚊科：双翅目）的自体发育

Autogeny in Culiseta longiareolata (Culicidae: Diptera) mosquitoes in laboratory conditions in Iran.

作者信息

Khaligh Fereshteh Ghahvechi, Naghian Abdollah, Soltanbeiglou Shadiyeh, Gholizadeh Saber

机构信息

Cellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran.

Medical Entomology Department, School of Public Health, Urmia University of Medical Sciences, Urmia, Iran.

出版信息

BMC Res Notes. 2020 Feb 19;13(1):81. doi: 10.1186/s13104-020-04942-5.

DOI:10.1186/s13104-020-04942-5

PMID:32075679

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7031961/

Abstract

OBJECTIVES

Culiseta longiareolata is a cosmopolitan species and has implicated in the transmission of avian malaria, tularemia, and arboviruses. Despite the wide distribution of Cs. longiareolata in Iran, little is known about its biology and physiology. The current research was conducted to study the autogeny behavior in this potential vector. During 2018, larvae and pupae were collected from Nazloo region in Urmia City using standard methods. Mosquitoes were reared in cages and fed by 5% sugar in laboratory conditions and were then dissected in phosphate-buffered saline (PBS) under a stereo microscope.

RESULTS

In total, 230 adult female Cs. longiareolata mosquitoes were dissected. Egg rafts were observed in the ovary of only 10.86% unfed female mosquitoes. Autogeny behavior is a significant factor in the growth of population without a blood feeding. Therefore, it is necessary to study how autogenous reproduction affects mosquito-borne diseases.

摘要

目的

长跗库蚊是一种世界性分布的物种，与禽疟疾、兔热病和虫媒病毒的传播有关。尽管长跗库蚊在伊朗分布广泛，但其生物学和生理学方面却鲜为人知。当前的研究旨在探究这种潜在病媒的自体生殖行为。2018年期间，使用标准方法从乌尔米耶市的纳兹卢地区采集幼虫和蛹。将蚊子饲养在笼子里，在实验室条件下用5%的糖水喂养，然后在体视显微镜下于磷酸盐缓冲盐水（PBS）中解剖。