Department of Medical Sciences, University of Turin, Turin, Italy.
Cell Stress Chaperones. 2013 May;18(3):353-7. doi: 10.1007/s12192-012-0388-5. Epub 2012 Dec 2.
The metabolic syndrome (MetS) confers an increased risk of both type 2 diabetes and cardiovascular diseases (CVD). Heat shock protein 70 (Hsp70), an intracellular polypeptide, can be exposed on the plasma membrane and/or released into the circulation, eliciting both native and immune responses that may contribute to vascular damage. Our aim was to assess if serum anti-Hsp70 antibody levels were cross-sectionally associated with uncomplicated MetS. A cross-sectional case-control study from the nondiabetic cohort of the Casale Monferrato Study was performed. Subjects with established CVD and/or abnormal renal function were excluded. Case subjects (n = 180) were defined as those fulfilling the criteria for the diagnosis of MetS. Control subjects (n = 136) were completely free of any component of the MetS. Serum anti-Hsp70 levels were measured by immunoenzymatic assay. We found that anti-Hsp70 antibody levels were significantly higher in cases than in control subjects [122.6 (89.5-155.6) vs 107.1 (77.3-152.4) μg/ml, p = 0.04], even after age and sex adjustment. In logistic regression analysis, higher levels of log-anti-Hsp70 conferred greater odds ratio (OR) for MetS, independently of age and sex. There was a statistically significant trend of ORs across quartiles of anti-Hsp70 and values greater than 108.0 μg/ml conferred a 77% increased OR of MetS as compared with values in the lower quartiles. The strength of the association slightly decreased after further adjustment for apolipoprotein B, smoking, and albumin excretion rate. In conclusion, our results show that serum anti-Hsp70 antibody levels are independently associated with nascent MetS.
代谢综合征（MetS）会增加 2 型糖尿病和心血管疾病（CVD）的风险。热休克蛋白 70（Hsp70）是一种细胞内多肽，可暴露于质膜上和/或释放到循环中，引发固有和免疫反应，从而导致血管损伤。我们的目的是评估血清抗 Hsp70 抗体水平是否与单纯性 MetS 存在横断面相关性。这是一项来自 Casale Monferrato 研究的非糖尿病队列的病例对照研究。患有已确诊 CVD 和/或肾功能异常的患者被排除在外。病例组（n = 180）被定义为符合 MetS 诊断标准的患者。对照组（n = 136）完全没有任何 MetS 成分。通过免疫酶联测定法测量血清抗 Hsp70 水平。我们发现，病例组的抗 Hsp70 抗体水平明显高于对照组[122.6（89.5-155.6）vs 107.1（77.3-152.4）μg/ml，p = 0.04]，即使在年龄和性别调整后也是如此。在逻辑回归分析中，较高水平的 log-抗 Hsp70 独立于年龄和性别赋予更大的比值比（OR）用于 MetS。抗 Hsp70 四分位间距的 OR 呈统计学显著趋势，与较低四分位数相比，值大于 108.0 μg/ml 时 MetS 的 OR 增加了 77%。在进一步调整载脂蛋白 B、吸烟和白蛋白排泄率后，关联的强度略有下降。总之，我们的研究结果表明，血清抗 Hsp70 抗体水平与新生的 MetS 独立相关。
