Laboratory of Bioorganic Chemistry, NIDDK, NIH , DHHS, Bethesda, Maryland 20892, United States.
Anal Chem. 2013 Jan 2;85(1):251-7. doi: 10.1021/ac3026459. Epub 2012 Dec 18.
We report the development of chemically modified peptide nucleic acids (PNAs) as probes for qualitative and quantitative detection of DNA. The remarkable stability of PNAs toward enzymatic degradation makes this class of molecules ideal to develop as part of a diagnostic device that can be used outside of a laboratory setting. Using an enzyme-linked reporter assay, we demonstrate that excellent levels of detection and accuracy for anthrax DNA can be achieved using PNA probes with suitable chemical components designed into the probe. In addition, we report on DNA-templated cross-linking of PNA probes as a way to preserve genetic information for repetitive and subsequent analysis. This report is the first detailed examination of the qualitative and quantitative properties of chemically modified PNA for nucleic acid detection and provides a platform for studying and optimizing PNA probes prior to incorporation into new technological platforms.
我们报告了化学修饰的肽核酸（PNA）作为定性和定量检测 DNA 的探针的发展。PNA 对酶降解的稳定性使得这类分子非常适合开发成为诊断设备的一部分，可以在实验室环境之外使用。通过酶联报告测定，我们证明了使用具有适当化学组成的 PNA 探针可以实现炭疽 DNA 的出色检测水平和准确性。此外，我们还报告了 PNA 探针的 DNA 模板交联作为一种保存遗传信息的方法，以便进行重复和后续分析。本报告首次详细检查了化学修饰 PNA 用于核酸检测的定性和定量特性，并为在纳入新技术平台之前研究和优化 PNA 探针提供了一个平台。
