Ocular Pharmacology Laboratory, Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences & Research, University of Delhi, New Delhi, India-110017.
Vascul Pharmacol. 2012 Nov-Dec;57(5-6):201-7. doi: 10.1016/j.vph.2012.02.007. Epub 2012 Feb 21.
The purpose of the study was to evaluate vasculoprotective effects of Hesperetin (Hsp) in Streptozotocin induced diabetic rats. The study was carried out for a period of 24weeks and evaluated for angiogenic parameters (VEGF and PKC-β), retinal vascular leakage by fluorescein angiography and, vessel (arteriolar and venular) diameters and any morphological abnormality through fundus photographs. Apart from this, transmission electron microscopy (TEM) was done to determine capillary basement membrane (BM) thickness. The results of the present study showed a significant increase in the expression of VEGF and PKC-β in diabetic retinae as compared to normal retinae. On the other hand, Hsp-treated retinae showed marked inhibition in the expression of VEGF and PKC-β. In the present study, diabetic retinae showed increase vascular permeability and leakage as compared to normal retinae. However, Hsp-treated retinae have not shown any such vascular dysfunctions. Moreover, there was significant increase in vessel caliber recorded in diabetic retinae compared to normal retinae, on the contrary Hsp-treated retinae showed lesser dilated vessels. Further, TEM study showed thickened BM in diabetic group as compared to normal group. However, Hsp-treated retinae showed marked prevention in BM thickness. In conclusion, it can be sated that Hsp has potential vasoprotective effects and can be useful in preventing diabetes induced vasculopathy.
本研究旨在评估橙皮苷（Hsp）对链脲佐菌素诱导的糖尿病大鼠的血管保护作用。该研究进行了 24 周，并评估了血管生成参数（VEGF 和 PKC-β）、荧光素血管造影的视网膜血管渗漏以及通过眼底照片评估血管（小动脉和小静脉）直径和任何形态异常。除此之外，还通过透射电子显微镜（TEM）来确定毛细血管基底膜（BM）的厚度。本研究结果表明，与正常视网膜相比，糖尿病视网膜中 VEGF 和 PKC-β 的表达显著增加。另一方面，Hsp 处理的视网膜中 VEGF 和 PKC-β 的表达明显受到抑制。在本研究中，与正常视网膜相比，糖尿病视网膜表现出增加的血管通透性和渗漏。然而，Hsp 处理的视网膜没有表现出任何这种血管功能障碍。此外，与正常视网膜相比，糖尿病视网膜的血管口径明显增加，相反，Hsp 处理的视网膜显示出较小的扩张血管。此外，TEM 研究表明，糖尿病组的 BM 比正常组厚。然而，Hsp 处理的视网膜明显预防了 BM 厚度的增加。总之，可以说 Hsp 具有潜在的血管保护作用，可用于预防糖尿病引起的血管病变。
