Bramwell H, Cass A E, Gibbs P N, Green M J
Centre for Biotechnology, Imperial College of Science, Technology & Medicine, London, UK.
Analyst. 1990 Feb;115(2):185-8. doi: 10.1039/an9901500185.
A method is proposed for the determination of paracetamol in whole undiluted blood, based on the enzymatic hydrolysis of the drug to p-aminophenol, which is then measured by chronoamperometry at a glassy carbon electrode. Hydrolysis of the paracetamol prior to electro-oxidation is shown to alleviate problems that arise from high background currents in the whole blood and so ensures a good linear correlation (r greater than 0.99) between the current and the paracetamol concentration. Recovery experiments and comparison with a reference method based on spectrophotometry suggest that the electrochemical assay only measures that proportion of paracetamol that is not bound to serum albumin.
提出了一种测定全血中对乙酰氨基酚的方法，该方法基于药物酶解为对氨基酚，然后通过计时电流法在玻碳电极上进行测定。对乙酰氨基酚在电氧化前进行水解可减轻全血中高背景电流引起的问题，从而确保电流与对乙酰氨基酚浓度之间具有良好的线性相关性（r大于0.99）。回收率实验以及与基于分光光度法的参考方法的比较表明，电化学测定法仅测量未与血清白蛋白结合的那部分对乙酰氨基酚。
