从杏鲍菇的子实体中分离得到一种新型甘露半乳糖聚糖的结构阐明。
Structural elucidation of a novel mannogalactan isolated from the fruiting bodies of Pleurotus geesteranus.
机构信息
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, People's Republic of China.
出版信息
Carbohydr Polym. 2013 Jan 30;92(1):236-40. doi: 10.1016/j.carbpol.2012.08.105. Epub 2012 Sep 1.
A novel water-soluble polysaccharide named PGPB-1, with a molecular weight of 1.3 × 10(4)Da as determined by high-performance liquid chromatography (HPLC), was isolated from the fruiting bodies of Pleurotus geesteranus by boiling water as well as DEAE-Sepharose Fast Flow and High-Resolution Sephacryl S-300. On the bases of compositional analysis, methylation analysis and NMR spectroscopy ((1)H NMR, (13)C NMR, 2D-COSY, TOCSY, HMQC, HMBC and NOESY spectra) analysis, the structural elucidation of PGPB-1 consisted of a α-D-(1→6)-galactopyranan and a α-D-3-O-Me-D-galactosyl unit backbone with a α-D-Mannosyl unit on O-2 of the 2,6-di-O-substituted-D-galactosyl units. The repeating unit of the polysaccharide was established as: [formula in text] PGPB-1 also contains a minor proportion of 6-linked-D-Glcp.
从金顶侧耳（Pleurotus geesteranus）的子实体中提取到一种新型水溶性多糖 PGPB-1，通过高效液相色谱（HPLC）确定其分子量为 1.3×10(4)Da。该多糖可通过沸水提取以及 DEAE-Sepharose Fast Flow 和 High-Resolution Sephacryl S-300 进一步分离。根据组成分析、甲基化分析和 NMR 光谱（（1）H NMR、（13）C NMR、2D-COSY、TOCSY、HMQC、HMBC 和 NOESY 谱）分析，PGPB-1 的结构由α-D-(1→6)-半乳糖吡喃聚糖和α-D-3-O-Me-D-半乳糖基单元主链以及 2,6-二-O-取代-D-半乳糖基单元上的α-D-甘露糖基单元组成。多糖的重复单元为：[文中公式]PGPB-1 还含有少量的 6-连接-D-Glcp。
Zotero 插件