College of Life Science, Shandong Agricultural University, Taian, 271018, P. R. China.
College of Chemistry, Nanjing Tech University, Nanjing, 211816, P. R. China.
Sci Rep. 2018 Jul 12;8(1):10493. doi: 10.1038/s41598-018-28785-0.
The present work investigated the hepatoprotective role of exopolysaccharides (EPS) isolated from the mushroom Pleurotus geesteranus with respect to alcohol-induced liver injury in mice. Based on a physico-chemical analysis, the EPS produced by Pleurotus geesteranus was identified as a heteropolysaccharide with α-glycosidic bond. The results revealed that prophylactic application of the EPS reduces detrimental alcoholic effects on the liver. This observation was followed by decreased levels of total cholesterol, triglycerides, CYP2E1 and pro-inflammatory mediators (TNF-α, IL-6, IL-1β, COX-2, NO and iNOS) in the liver homogenates, suggesting that the EPS exhibits anti-inflammatory and hepatoprotective effects. Moreover, the increased activity of hepatic enzymes (superoxide dismutase, glutathione peroxidase and catalase) and reduced lipid peroxidation status indicated that the antioxidative effect of the EPS contributes to alleviation of liver injury. Therefore, this study reports that the EPS produced by Pleurotus geesteranus could be considered a potential natural drug or functional food supplement for the prevention of liver damage.
本研究旨在探讨从蘑菇糙皮侧耳中提取的胞外多糖（EPS）对小鼠酒精性肝损伤的保护作用。基于理化分析，鉴定糙皮侧耳 EPS 为具有α-糖苷键的杂多糖。结果表明，EPS 的预防性应用可降低酒精对肝脏的有害影响。这一观察结果伴随着肝匀浆中总胆固醇、甘油三酯、CYP2E1 和促炎介质（TNF-α、IL-6、IL-1β、COX-2、NO 和 iNOS）水平的降低，表明 EPS 具有抗炎和保肝作用。此外，肝酶（超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶）活性的增加和脂质过氧化状态的降低表明 EPS 的抗氧化作用有助于减轻肝损伤。因此，本研究表明糙皮侧耳 EPS 可被视为预防肝损伤的潜在天然药物或功能性食品补充剂。
