Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Talanta. 2012 Aug 30;98:226-30. doi: 10.1016/j.talanta.2012.06.078. Epub 2012 Jul 3.
A new method for determination of L-tryptophan (L-try) using the flow injection chemiluminescence (FI-CL) system of KMnO(4)-SnCl(2)-CHOH based on a graphene oxide-magnetite-molecularly imprinted polymer (GM-MIP) is described. The L-try GM-MIP was synthesized using graphene oxide (G) which improved the adsorption capacity as carrier, and magnetite nanoparticles which made the polymers easier to use in the sensor. The adsorption performance and properties were characterized. The GM-MIP was used in CL analysis to increase the selectivity and the possible mechanism was also discussed. The CL sensor responded linearly to the concentration of L-try over the range from 2.10×10(-7) to 7.09×10(-4) M with a detection limit of 2.11×10(-8) M (3σ). The relative standard deviation (RSD) for the determination of 3.0×10(-5) M L-try was 2.40% (n=11). On the basis of speediness and sensitivity, the sensor is reusable and shows a great improvement in selectivity and adsorption capacity over other sensors. The sensor has been used for the determination of L-try in drug samples.
一种基于氧化石墨烯-磁铁矿-分子印迹聚合物（GM-MIP）的新方法，利用 KMnO(4)-SnCl(2)-CHOH 流动注射化学发光（FI-CL）系统测定 L-色氨酸（L-try）。采用氧化石墨烯（G）作为载体，提高了吸附能力，采用磁铁矿纳米粒子，使聚合物更易于在传感器中使用，合成了 L-try GM-MIP。对 GM-MIP 的吸附性能和特性进行了表征。将 GM-MIP 用于 CL 分析，以提高选择性，并讨论了可能的机制。CL 传感器对 L-try 的浓度在 2.10×10(-7)到 7.09×10(-4) M 范围内呈线性响应，检测限为 2.11×10(-8) M（3σ）。测定 3.0×10(-5) M L-try 的相对标准偏差（RSD）为 2.40%（n=11）。基于速度和灵敏度，该传感器可重复使用，在选择性和吸附能力方面优于其他传感器。该传感器已用于药物样品中 L-try 的测定。
