Genètica i Millora Animal, IRTA, Lleida, 25198, Spain.
Gene. 2013 Feb 15;515(1):220-3. doi: 10.1016/j.gene.2012.11.029. Epub 2012 Dec 7.
Solute-carrier family 27A molecules are integral transmembrane proteins that play a fundamental role in the uptake of long-chain fatty acids into mammalian cells. Our goal was to characterize this multigene family in pigs. Chromosomal location of the six porcine SLC27A genes was determined by radiation hybrid mapping and indicated that the six genes map to six different chromosomal locations. Moreover, we analyzed SLC27A mRNA expression in six pig tissues by quantitative RT-PCR. While SLC27A1, SLC27A3 and SLC27A4 were expressed in most, if not all, analyzed tissues, SLC27A2, SLC27A5 and SLC27A6 were predominantly expressed in the liver. In general, pig and human SLC27A mRNA expression profiles were remarkably concordant, although important differences were observed for SLC27A1 and SLC27A6 mRNAs. Discrepancies between mRNA expression profiles have been observed even in closely related primate species, and they might reflect the acquisition of regulatory changes promoting evolutionary adaptation.
溶质载体家族 27A 分子是整合跨膜蛋白，在长链脂肪酸进入哺乳动物细胞的过程中发挥着重要作用。我们的目标是鉴定猪中的这个多基因家族。通过放射杂交作图确定了 6 个猪 SLC27A 基因的染色体位置，表明这 6 个基因位于 6 个不同的染色体位置。此外，我们通过定量 RT-PCR 分析了 6 种猪组织中的 SLC27A mRNA 表达。虽然 SLC27A1、SLC27A3 和 SLC27A4 在大多数（如果不是全部）分析组织中表达，但 SLC27A2、SLC27A5 和 SLC27A6 主要在肝脏中表达。一般来说，猪和人类 SLC27A mRNA 表达谱非常一致，尽管 SLC27A1 和 SLC27A6 mRNA 存在重要差异。即使在密切相关的灵长类物种中，也观察到了 mRNA 表达谱的差异，这些差异可能反映了促进进化适应的调节变化的获得。
