Weinreb R N, Dreher A W, Coleman A, Quigley H, Shaw B, Reiter K
Department of Ophthalmology, University of California, San Diego, La Jolla 92093.
Arch Ophthalmol. 1990 Apr;108(4):557-60. doi: 10.1001/archopht.1990.01070060105058.
We describe a new technique for the measurement of retinal nerve fiber layer thickness and compare its results with histopathologic measurements in the same eyes. For these studies, two fixed monkey eyes were incised and placed on a pedestal in a plastic viewing dish. The eyes were perfused to maintain a pressure between 10 and 20 mm Hg. An ellipsometer, an optical device used to measure the change in polarization of light (retardation), was implemented in a laser tomographic scanner to obtain polarization data from the two monkey retinas. For the 15 measured locations, retardation ranged between a mean (+/- SD) of 0.9 degrees +/- 1.8 degrees and 23.7 degrees +/- 0.3 degrees. Subsequently, retinal nerve fiber layer thickness was measured at the imaged points in epoxy resin-embedded sections by an observer masked to the ellipsometry data. These values ranged between 20.4 microns and 213.9 microns. There was an excellent correlation (R = .83) between retardation and the histopathologic measurement of retinal nerve fiber layer thickness. Quantitating retinal nerve fiber layer thickness may enhance discrimination between glaucomatous and normal eyes earlier than is currently available by anatomic and functional approaches.
我们描述了一种测量视网膜神经纤维层厚度的新技术，并将其结果与同一只眼睛的组织病理学测量结果进行比较。在这些研究中，将两只固定的猴眼切开，放置在塑料观察盘中的基座上。对眼睛进行灌注以维持10至20毫米汞柱之间的压力。在激光断层扫描仪中使用椭圆偏振仪（一种用于测量光偏振变化（相位延迟）的光学装置）从两只猴视网膜获取偏振数据。对于15个测量位置，相位延迟范围在平均（±标准差）0.9度±1.8度至23.7度±0.3度之间。随后，由对椭圆偏振测量数据不知情的观察者在环氧树脂包埋切片的成像点处测量视网膜神经纤维层厚度。这些值在20.4微米至213.9微米之间。相位延迟与视网膜神经纤维层厚度的组织病理学测量之间存在极好的相关性（R = 0.83）。定量测量视网膜神经纤维层厚度可能比目前通过解剖学和功能方法更早地增强青光眼和正常眼之间的鉴别能力。
