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在挪威云杉防御针对 H. annosum s.l. 的过程中,主要的模块似乎是茉莉酸介导的信号转导,而不是水杨酸介导的信号转导的拮抗作用。

The primary module in Norway spruce defence signalling against H. annosum s.l. seems to be jasmonate-mediated signalling without antagonism of salicylate-mediated signalling.

机构信息

Department of Forest Mycology and Plant Pathology, Uppsala Biocenter, Swedish University of Agricultural Sciences, Uppsala, Sweden.

出版信息

Planta. 2013 Apr;237(4):1037-45. doi: 10.1007/s00425-012-1822-8. Epub 2012 Dec 8.

Abstract

A key tree species for the forest industry in Europe is Norway spruce [Picea abies (L.) Karst.]. One of its major diseases is stem and butt rot caused by Heterobasidion parviporum (Fr.) Niemelä & Korhonen, which causes extensive revenue losses every year. In this study, we investigated the parallel induction of Norway spruce genes presumably associated with salicylic acid- and jasmonic acid/ethylene-mediated signalling pathways previously observed in response to H. parviporum. Relative gene expression levels in bark samples of genes involved in the salicylic acid- and jasmonic acid/ethylene-mediated signalling pathways after wounding and inoculation with either the saprotrophic biocontrol fungus Phlebiopsis gigantea or with H. parviporum were analysed with quantitative PCR at the site of the wound and at two distal locations from the wound/inoculation site to evaluate their roles in the induced defence response to H. parviporum in Norway spruce. Treatment of Norway spruce seedlings with methylsalicylate, methyljasmonate and inhibitors of the jasmonic acid/ethylene signalling pathway, as well as the Phenylalanine ammonia lyase inhibitor 2-aminoindan-2-phosphonic acid were conducted to determine the responsiveness of genes characteristic of the different pathways to different hormonal stimuli. The data suggest that jasmonic acid-mediated signalling plays a central role in the induction of the genes analysed in this study irrespective of their responsiveness to salicylic acid. This may suggest that jasmonic acid-mediated signalling is the prioritized module in the Norway spruce defence signalling network against H. parviporum and that there seems to be no immediate antagonism between the modules in this interaction.

摘要

欧洲森林产业的主要树种之一是挪威云杉 [Picea abies (L.) Karst.]。它的主要病害之一是由 Heterobasidion parviporum(Fr.)Niemelä & Korhonen 引起的茎干和基腐病,每年都会造成广泛的收入损失。在这项研究中,我们研究了挪威云杉基因的平行诱导,这些基因可能与之前观察到的水杨酸和茉莉酸/乙烯介导的信号通路有关,这些信号通路是对 H. parviporum 反应的结果。在树皮样本中,用定量 PCR 分析了水杨酸和茉莉酸/乙烯介导的信号通路中参与基因的相对表达水平,这些基因涉及创伤后和接种腐生生物防治真菌 Phlebiopsis gigantea 或 H. parviporum 时的基因表达。在创伤部位和创伤/接种部位的两个远端位置分析基因表达水平,以评估它们在挪威云杉对 H. parviporum 诱导防御反应中的作用。用水杨酸甲酯、茉莉酸甲酯和茉莉酸/乙烯信号通路抑制剂以及苯丙氨酸解氨酶抑制剂 2-氨基茚-2-磷酸处理挪威云杉幼苗,以确定不同途径特征基因对不同激素刺激的反应性。数据表明,茉莉酸介导的信号转导在本研究中分析的基因的诱导中起核心作用,而与它们对水杨酸的反应性无关。这可能表明,茉莉酸介导的信号转导是挪威云杉防御信号网络针对 H. parviporum 的优先模块,而且在这种相互作用中,模块之间似乎没有直接的拮抗作用。

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