Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai 200031, China.
J Biomed Mater Res A. 2013 May;101(5):1511-7. doi: 10.1002/jbm.a.34444. Epub 2012 Dec 7.
Silk fibroin (SF) shows promise for tissue engineering and other biomedical applications due to its excellent biocompatibility, unique biomechanical properties, and controllable biodegradability. The particulate form of SF materials may have many potential uses, including the use as a filler for tissue defects or as a controlled-release agent for drug delivery. However, many past in vivo and in vitro studies evaluating the biocompatibility and biodegradability of SF have involved bulk implants. It is essential to evaluate the inflammatory effects of SF particles before further use. In this study, two different sizes of SF particles were evaluated to assess their impact on the release of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6, in comparison with lipopolysaccharide positive control stimulation. The inflammatory processes were characterized using real-time reverse transcription polymerase chain reaction, enzyme-linked immunosorbent assay, and light microscopy evaluations. The results indicated that small silk fibroin particles and large silk fibroin particles, in culture with RAW 264.7 murine macrophage cells for 24 h, caused up-regulation of mRNA coding for TNF-α, which indicated that both size of particles have potential inflammatory effects. There was a statistically significant increase in this up-regulation under small silk fibroin stimulation. However, the immunosorbent assay suggested that there was virtually no observed release of IL-1β, IL-6, or TNF-α, relative to the control group. The results suggest that SF particles of the chosen dimensions may have good biocompatibility in culture with RAW 264.7 murine macrophages.
丝素蛋白（SF）由于其优异的生物相容性、独特的生物机械性能和可控制的生物降解性，在组织工程和其他生物医学应用中具有广阔的应用前景。SF 材料的颗粒形式可能有许多潜在的用途，包括用作组织缺陷的填充剂或用作药物递送的控释剂。然而，许多过去评估 SF 的生物相容性和生物降解性的体内和体外研究都涉及大块植入物。在进一步使用之前，评估 SF 颗粒的炎症反应是至关重要的。在这项研究中，评估了两种不同尺寸的 SF 颗粒，以评估它们对肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β和 IL-6 释放的影响，与脂多糖阳性对照刺激进行比较。使用实时逆转录聚合酶链反应、酶联免疫吸附测定和荧光显微镜评估来描述炎症过程。结果表明，小丝素蛋白颗粒和大丝素蛋白颗粒在与 RAW 264.7 鼠巨噬细胞共培养 24 小时后，导致 TNF-α编码的 mRNA 上调，这表明两种颗粒尺寸都具有潜在的炎症作用。在小丝素蛋白刺激下，这种上调有统计学意义的增加。然而，免疫吸附测定表明，与对照组相比，几乎没有观察到 IL-1β、IL-6 或 TNF-α的释放。结果表明，在所选择的尺寸范围内的 SF 颗粒在与 RAW 264.7 鼠巨噬细胞共培养时可能具有良好的生物相容性。
